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Series GSE6106 Query DataSets for GSE6106
Status Public on Nov 07, 2006
Title Sense-antisense transcript comparison in mouse brain and kidney
Organism Mus musculus
Experiment type Expression profiling by array
Summary Comparison of sense (forward probes) and antisense (reverse probes on U74 v1 gene arrays) transcripts in mouse kidney and brain.
Positive calls related to antisense transcripts were compared to the cognate signals on the 430 version of mouse genome arrays to obtain genes that co expressed sense and antisense transcripts. This had to be done manually because divergent probe IDs on the two chip generations.
Keywords: Qualitative comparison of expression
Overall design The first Affymetrix U74 mouse gene chips contains reversely oriented probe sets. These probes will hybridize to natural antisense transcripts that overlap with the sense transcript in the cognate area. Affymetrix provided a mask to identify the reverse probes. Positive calls with these reverse probes will give an estimate of the antisense transcriptome in that particular tissue. Comparison of the reversely oriented probes with correctly annotated probes will reveal the expression ratio of sense and antisense transcripts.
Contributor(s) Peters H, Jackson MS, Lastowska MA, Werner A
Citation(s) 17105753
Submission date Oct 23, 2006
Last update date Feb 11, 2019
Contact name Andreas Werner
Phone +44 191 222 6990
Organization name Newcastle University
Department Cell and Molecular Biosciences
Street address Framlington Place
City Newcastle
ZIP/Postal code NE2 4HH
Country United Kingdom
Platforms (5)
GPL32 [MG_U74A] Affymetrix Murine Genome U74A Array
GPL33 [MG_U74B] Affymetrix Murine Genome U74B Array
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (7)
GSM141677 mouse brain antisense U74A
GSM141678 mouse brain antisense U74B
GSM141680 mouse kidney antisense U74A
BioProject PRJNA97575

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6106_RAW.tar 17.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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