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Series GSE6110 Query DataSets for GSE6110
Status Public on Aug 30, 2007
Title Increased Susceptibility of Aging Kidney to Ischemic Injury: Identification of Molecular Pathways using Microarray
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Aging is associated with an increased incidence and severity of acute renal failure. However, the molecular mechanism(s) underlying the increased susceptibility to injury remain undefined. These experiments were designed to investigate the influence of age on the response of the kidney to ischemic and nephrotoxic challenge. Renal slices were prepared from young (4 month), aged ad libitum (aged-AL; 24 month) and aged caloric restricted (aged-CR; 24 month) male Fischer 344 rats and subjected to ischemic stress (anoxia, 100% N2) for 0-60 min and to cisplatin (2 mM, 4hr) challenge. As assessed by biochemical and histological evaluation, slices from aged-AL rats were more susceptible to injury than young counterparts. Importantly, caloric restriction attenuated the increased susceptibility to injury. In an attempt to identify the molecular pathway(s) underlying this response, microarray analysis was performed on tissue harvested from the same animals used for the functional analysis. RNA was isolated and the corresponding cDNA was hybridized to CodeLinkā„¢ Rat Whole Genome Bioarray slides. Subsequent gene expression analysis was performed using GeneSpring software. Using two-sample t-tests and a 2-fold cut-off, the expression of 92 genes was changed during aging and attenuated by caloric restriction. In summary, several changes were identified that may be associated with the increased susceptibility of aging kidney to injury.
Keywords: aging, caloric-restriction, kidney
Overall design This study utilized three groups (young, aged ad libitum, and aged caloric-restricted) each with 4 biological replicates for a total of 12 samples. Each sample was hybridized to a single one-color array platform with no reference samples being used for a total of 12 arrays.
Contributor(s) Chen G, Bridenbaugh EA, Zawieja DC, Burghardt RC, Parrish AR
Citation(s) 17670906
Submission date Oct 23, 2006
Last update date Mar 16, 2012
Contact name Alan R. Parrish
Phone 979-458-1538
Organization name Texas A&M Health Science Center
Department Systems Biology
Street address 364 Reynolds
City College Station
State/province TX
ZIP/Postal code 77843-1114
Country USA
Platforms (1)
GPL4478 GE Healthcare/Amersham Biosciences CodeLinkā„¢ Rat Whole Genome Bioarray
Samples (12)
GSM141699 Kidney aged CR rep1
GSM141700 Kidney aged CR rep2
GSM141701 Kidney aged CR rep3
BioProject PRJNA97717

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Supplementary file Size Download File type/resource
GSE6110_RAW.tar 27.9 Mb (http)(custom) TAR (of XLS)

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