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| Status |
Public on Nov 17, 2014 |
| Title |
High throughput quantitative whole transcriptome analysis of CC10- B4+ and Krt5-CreERT2 - labeled distal lung cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used microfluidic single cell RNA-seq on adult isolated CC10-CreERT2 (negative) integrin beta4(pos) cells lung epithelial cells in order to determine the transcriptional profile of this putative progenitor population.
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| Overall design |
CC10-CreERT2 / tdTomato (negative) integrin beta4(pos) cells were isolated by FACS, as were Krt5-CreERT2 / tdTomato (positive) cells. These cells were pooled and loaded onto the Fluidigm C1 device.
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| Contributor(s) |
Vaughan AE, Chapman HA |
| Citation(s) |
25533958 |
| Submission date |
Sep 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Vaughan |
| E-mail(s) |
andrewva@vet.upenn.ed
|
| Phone |
2062939309
|
| Organization name |
University of Pennsylvania
|
| Street address |
3800 Spruce Street
|
| City |
PHILADELPHIA |
| State/province |
United States |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA260708 |
| SRA |
SRP046995 |
