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Series GSE6134 Query DataSets for GSE6134
Status Public on Aug 08, 2007
Title Offsprings of crosses between hypercholesterolemic and normocholesterolemic parents LUMC-HKG-ApoE-Atherosclerosis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Enhanced prenatal fatty streak formation in human fetuses has been associated with maternal hypercholesterolemia. However, the possible roles of maternal genetic background and in utero environment on development of atherosclerosis in adult life have not been unraveled. We generated genetically identical heterozygous apoE-deficient mice offspring with a different maternal background to study the intrauterine effect of maternal genotype and associated hypercholesterolemia on the developing vascular system. As read out for increased atherosclerosis development in adult life, a constrictive collar was placed around the carotid artery to induce lesion formation. A significant increase in endothelial cell activation and damage was detected in the carotid arteries of heterozygous apoE-deficient fetuses with apoE-deficient mothers compared with offspring from wild type mothers, but no fatty streak formation was observed. Postnatally, all carotid arteries revealed normal morphology. In adult offspring with maternal apoE-deficiency, the constrictive collar resulted in severe lesion (9/10) development compared with no to only minor lesions (2/10) in offspring of wild type mothers. Microarray analysis showed no effect of maternal apoE-deficiency on gene expression in adult offspring. We conclude that maternal apoE-deficiency not only affects fetal arteries, but also increases the susceptibility for development of collar-induced atherosclerosis in adult life.
Keywords: Atheroslcersosis development
 
Overall design Crossing of hypercholesterolemic female with normocholesterolemic male and vice versa, determination of susceptabilioty to atherosclerosis in offspring
 
Contributor(s) Alkemade FE, Gittenberg-de Groot AC, Schiel AE, vanMunsteren CJ, Havekes LM, Willems van Dijk K, de Ruiter MC
Citation(s) 17656671
Submission date Oct 25, 2006
Last update date Feb 11, 2019
Contact name Anja Schiel
E-mail(s) anja.schiel@medisin.uio.no
Organization name Leiden University Medical Center
Department Human Clinical Genetics
Lab Frants
Street address Einthovenweg 20
City Leiden
ZIP/Postal code 2300RC
Country Netherlands
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (7)
GSM142367 ApoE-/+, offspring normocholesterolemic wt mother, Apo-/- father LUMC_HKG_MOUSE_430_2
GSM142369 ApoE-/+, offspring normocholesterolemic wt mother, ApoE-/- father LUMC_HKG_MOUSE_430_2-2
GSM142370 ApoE-/+, offspring normocholesterolemic wt mother, ApoE-/- father LUMC_HKG_MOUSE_430_2-3
Relations
BioProject PRJNA97577

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6134_RAW.tar 25.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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