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| Status |
Public on Sep 18, 2014 |
| Title |
Expression data from HTR-8/Svneo side population (SP) and non-side population (NSP) cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Immature cell populations, including stem cells and progenitor cells, can be found in “side-population (SP)” cells. Although SP cells isolated from some adult tissues have been reported elsewhere, isolation and characterization of human trophoblast SP cells remained to be reported. We used microarrays to detail the global program of gene expression underlying cell differentiation and identified up-regulated genes of SP
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| Overall design |
HTR-8/SVneo SP cells or NSP cells were isolated using a FACS Vantage fluorescence-activated cell sorter (BD Bioscience, San Jose, California) using dual wavelength analysis (blue, 424 - 444nm; red 675 nm) after excitation with 350 nm UV light. PI-positive dead cells were excluded from the analysis. Sorted SP and NSP cells' RNA had been analyzed by microarray analysis.
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| Contributor(s) |
Kato K, Inagaki T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 18, 2014 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Tetsunori Inagaki |
| E-mail(s) |
tetsuina27@yahoo.co.jp
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| Organization name |
Juntendo University
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| Department |
Obstetrics and gynecology
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| Street address |
Hongo 2-1-1, Bunkyo-ku,
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| City |
Tokyo |
| ZIP/Postal code |
113-8431 |
| Country |
Japan |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA261438 |
