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| Status |
Public on Sep 20, 2014 |
| Title |
Mouse Bone-marrow Stromal Cell Lines Genetic Expression Comparison |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
OP9 stromal cells are strong inducers of mesoendodermal differentiation of human embryonic stem cells. They acquire inductive function after prolonged post-confluence culture. The experiment is designed to determine the genes specifically expressed in these highly inductive OP9 cells compared to non-confluent OP9 cultures as well as other stromal cell lines (MS5, S17).
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| Overall design |
OP9, MS5, and S17 cells were plated on gelatin-coated 10cm dishes in alphaMEM/20%FBS. Cells were collected at confluence (day 4) and after additional 4 days post-confluence culture. Half of the medium was replaced with fresh medium on day 4. Cells were collected by trypsin-EDTA, lysed in TRIReagent and strored at -80C up to 1 month. RNA was isolated and treated with DNase using Ambion RiboPure RNA isolation kit and TURBO DNAfree reagents. One sample was analyzed per condition.
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| Contributor(s) |
Slukvin I, Vodyanik M, Swanson S |
| Citation(s) |
25448067 |
| Submission date |
Sep 19, 2014 |
| Last update date |
Dec 22, 2014 |
| Contact name |
Igor Slukvin |
| E-mail(s) |
islukvin@wisc.edu
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| Phone |
608-263-0058
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| Organization name |
University of Wisconsin
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| Department |
Department of Pathology and Laboratory Medicine
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| Street address |
1220 Capitol Court
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL19213 |
NimbleGen Mus musculus 385K Array [2006_08_03_MM8_60mer_expr] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA261523 |
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