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| Status |
Public on Nov 02, 2014 |
| Title |
Identifying the targets of the pair-rule transcription factors Even-skipped and Runt in the late Drosophila blastoderm |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
It has long been appreciated that striped pair-rule transcription factor expression is necessary for convergent extension in the early Drosophila embryo, although the mechanisms that link these transcriptional regulators to planar polarity in this tissue have long been elusive. The goal of this study was to determine the transcriptional tragets of the pair-rule transcription factors Eve and Runt in Drosophila blastoderm embryos. We compared the transcriptional profiles of late blastoderm embryos injected with either water or dsRNAs against both eve and runt to identify differentially expressed genes that may directly contribute to the establishment of planar polarity during Drosophila convergent extension.
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| Overall design |
Comparing the mRNA profiles from late blastoderm Drosophila embryos injected with either water (Water) or eve+runt dsRNAs (Eve), in triplicate, using Illumina HiSeq.
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| |
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| Contributor(s) |
Pare AC, Zallen JA |
| Citation(s) |
25363762 |
| Submission date |
Sep 23, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Adam Paré |
| E-mail(s) |
parea@mskcc.org
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Developmental Biology
|
| Lab |
Jennifer Zallen
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA261837 |
| SRA |
SRP047433 |
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