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Series GSE61766 Query DataSets for GSE61766
Status Public on Sep 26, 2014
Title Expression data from rhesus macaque blood CD8+ T cells
Organism Macaca mulatta
Experiment type Expression profiling by array
Summary Immunization of macaques with simian immunodeficiency virus with deletions in nef (SIVΔnef) has been shown to elicit protective immunity to infection by pathogenic SIV, yet our understanding of the mechanisms that orchestrate protection and prevent pathogenesis remains limited. In the study, we utilize whole-genome transcriptional profiling to reveal molecular signatures of protective immunity in circulating CD8+ T cells of rhesus macaques vaccinated with SIVmac239Δnef and challenged with pathogenic SIVmac251.
Microarrays were used to characterize changes in gene expression in blood CD8+ T cells that occur following vaccination of rhesus macaques with attenuated SIV∆nef and subsequent challenge with pathogenic SIVmac251, in comparison to corresponding changes in healthy controls and unvaccinated animals infected with pathogenic SIVmac251
 
Overall design CD8+ T cells were isolated by magnetic beads from the blood of healthy uninfected macaques, macaques vaccinated with SIV∆nef, and unvaccinated controls infected with SIVmac251, and used for RNA extraction and hybridization on Affymetrix microarrays. Blood samples from vaccinated animals were collected prior to vaccination, at 3, 20, and 40 weeks following vaccination. After the 40 week vaccination period, macaques were challenged with SIVmac251, and blood was again collected at 3 weeks following challenge. Blood was collected from the unvaccinated controls at 3 weeks following infection with SIVmac251
 
Contributor(s) George MD
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Submission date Sep 25, 2014
Last update date Sep 27, 2014
Contact name Michael George
E-mail(s) mdgeorge@ucdavis.edu
Organization name University of California, Davis
Department Medical Microbiology and Immunology
Street address 451 Health Sciences Drive
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL3535 [Rhesus] Affymetrix Rhesus Macaque Genome Array
Samples (33)
GSM1513643 CD8+ T cells: healthy uninfected macaque biological rep 1
GSM1513644 CD8+ T cells: healthy uninfected macaque biological rep 2
GSM1513645 CD8+ T cells: healthy uninfected macaque biological rep 3
Relations
BioProject PRJNA262079

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Supplementary file Size Download File type/resource
GSE61766_RAW.tar 131.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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