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| Status |
Public on Jan 22, 2007 |
| Title |
Stress response of Saccharomyces cerevisiae to exposure to natural product pyocyanin. |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Pyocyanin has been shown to engage in redox transfer of electrons from NADPH to oxygen to generate superoxide radicals. Transcriptional response to oxygen stress has been characterized in yeast and should be observable upon exposure to pyocyanin if this is the true mode of action.
We used microarrays to detail the global programme of gene expression induced in the stress response to pyocyanin.
Keywords: stress response
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| Overall design |
Saccharomyces cerevisiae cells were exposed to 25 and 250 ug/ml of pyocyanin for a period of 6 hours incubation for RNA extraction and hybridization on Affymetrix microarrays. Results were compared to transcription levels in untreated cells under the same incubations conditions. The data indicated that transcriptional changes were consistent with an oxidative stress response, as observed by other researchers investigating the mode of action of pyocyanin toxicity.
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| Contributor(s) |
Watanabe CM, Angell S, Bench BJ, Williams H, Liu K |
| Citation(s) |
17185230 |
| Submission date |
Oct 27, 2006 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Coran MH Watanabe |
| E-mail(s) |
watanabe@mail.chem.tamu.edu
|
| Phone |
979-458-8094
|
| Fax |
979-458-8095
|
| Organization name |
Texas A*M University
|
| Department |
Chemistry
|
| Lab |
Watanabe
|
| Street address |
Texas A&M Dept. Chemistry MS 3255
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77843 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
|
| Samples (6)
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| GSM142982 |
yeastcells_control6hrsafterO.D.1.0_control1. |
| GSM142983 |
yeastcells_treatment6hrsafterO.D.1.0_25ug/ml T1 |
| GSM142984 |
yeastcells_control6hrsafterO.D.1.0_control2. |
| GSM142985 |
yeastcells_treatment6hrsafterO.D.1.0_25ug/ml T2 |
| GSM142986 |
yeastcells_treatment6hrsafterO.D.1.0_250ug/ml T1 |
| GSM142987 |
yeastcells_treatment6hrsafterO.D.1.0_250ug/ml T2 |
|
| Relations |
| BioProject |
PRJNA100657 |
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