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Series GSE61853 Query DataSets for GSE61853
Status Public on Mar 25, 2015
Title Gene expression analysis of bone marrow mesenchymal stromal cells from myelodysplastic syndrome (MDS) patients and normal controls
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Myelodysplastic syndrome (MDS) is a group of heterogeneous clonal stem cell disorders. We hypothesize that gene expression changes in the bone marrow (BM) microenvironment might play a fundamental role in the development and progression of MDS. The goal of the present study is to investigate the differences in gene expression profiles of BM mesenchymal stromal cells (MSCs) between MDS patients and normal individuals, as well as between MDS subtypes. To this end, we applied global gene expression profiling on BM MSCs from adult de novo MDS patients and from controls.
The results suggest that gene expression of the MDS BM microenvironment is difference from control BM. In particular, interferon signaling pathway was shown to be up-regulated in MDS BM. The results also showed altered expression according to disease progression. The present study provides evidence supporting MDS as an immune disease and suggests that BM MSCs are a possible therapeutic target in MDS.
 
Overall design The present study include seven adults referred for staging of lymphoma with no evidence of BM involvement as controls, and seven adults diagnosed as de novo MDS cases. The MDS patients consist of three RCMD, three RAEB-1, and one RAEB-2 (abbreviation, RCMD: refractory cytopenia with multilineage dysplasia; RAEB: refractory anemia with excess blasts). Total RNA was isolated from BM MSCs for gene expression analysis that compares the three groups - control (n=7), RCMD (n=3), and RAEB (n=4) - in a pairwise manner.
 
Contributor(s) Kim M, Lee DS
Citation(s) 25803272
Submission date Sep 29, 2014
Last update date Aug 13, 2018
Contact name Seungwoo Hwang
E-mail(s) swhwang10@yahoo.com
Phone 82-42-879-8544
Organization name Korea Research Institute of Bioscience and Biotechnology
Department Korean Bioinformation Center
Street address 52 Eoeun-dong Yuseong-gu
City Daejeon
ZIP/Postal code 305-806
Country South Korea
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (14)
GSM1515746 BM_MSCs-Control-rep1
GSM1515747 BM_MSCs-Control-rep2
GSM1515748 BM_MSCs-Control-rep3
Relations
BioProject PRJNA262556

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61853_RAW.tar 26.2 Mb (http)(custom) TAR
GSE61853_non-normalized.txt.gz 4.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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