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| Status |
Public on Dec 17, 2015 |
| Title |
Genome wide binding of glucocorticoid receptor in dexamethasone treated human monocyte derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Macrophages are amongst the major targets of glucocorticoids (GC) as therapeutic anti-inflammatory agents. Here we show that GC treatment of mouse and human macrophages initiates a cascade of induced gene expression including many anti-inflammatory genes. Inducible binding of the glucocorticoid receptor (GR) was detected at candidate enhancers in the vicinity of induced genes in both species and this was strongly associated with canonical GR binding motifs. However, the sets of inducible genes, the candidate enhancers, and the GR motifs within them, were highly-divergent between the two species.. The data cast further doubt upon the predictive value of mouse models of inflammatory disease.
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| Overall design |
human monocyte derived macrophages were treated with dexamethasone for 2h, fixed and ChIP performed. Material from 4 volunteers was pooled for the IP.
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| Contributor(s) |
Jubb AW, Bickmore WA, Hume DA, Young RS |
| Citation(s) |
26663721, 29241532 |
| Submission date |
Sep 29, 2014 |
| Last update date |
Jul 15, 2019 |
| Contact name |
Alasdair W Jubb |
| E-mail(s) |
alasdair.jubb@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Department |
Roslin Institute
|
| Lab |
Hume lab / Bickmore lab (MRC HGU)
|
| Street address |
The Roslin Institute
|
| City |
Easter Bush |
| State/province |
Midlothian |
| ZIP/Postal code |
EH25 9RG |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE61881 |
Divergent transcriptional activation by glucocorticoids in mouse and human macrophages is the result of gain and loss of enhancers |
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| Relations |
| BioProject |
PRJNA262586 |
| SRA |
SRP048473 |
