|
| Status |
Public on Mar 21, 2009 |
| Title |
Mouse Reissner's Membrane Gene Expression studies |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal was to screen for the expressed genes in Reissner's membrane (RM) that are related to ion transport and its regulation. The objectives were 1) to determine whether short-term incubation altered the transcriptome and 2 ) to discover which genes changed expression levels in response to glucocorticoids.
Keywords: drug response
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| |
|
| Overall design |
RM was dissected free-hand from mouse cochleae and either immediately subjected to RNA extraction or incubated for 24 as explants. Two series of samples were incubated in the presence or absence of dexamethasone (100 nM) and samples of RM were pooled to obtain sufficient material for gene array analysis. The pooled samples were used to hybridize 3 gene array chips for each biological sample.
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| |
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| Contributor(s) |
Marcus DC, Raveendran NN, Wu T |
| Citation(s) |
19144862, 23537040, 20226170 |
| Submission date |
Oct 30, 2006 |
| Last update date |
Sep 24, 2019 |
| Contact name |
Daniel Marcus |
| E-mail(s) |
marcus@vet.ksu.edu
|
| Phone |
785-532-4532
|
| Organization name |
Kansas State University
|
| Department |
Anatomy & Physiology
|
| Lab |
Cellular Biophysics lab
|
| Street address |
228 Coles Hall
|
| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (9)
|
| GSM142988 |
KSUAP-DM mReissners Membrane Dex24hrA |
| GSM143022 |
KSUAP-DM mReissners Membrane Dex24hrB |
| GSM143023 |
KSUAP-DM mReissners Membrane Dex24hrC |
| GSM143024 |
KSUAP-DM mReissners Membrane I24hrA |
| GSM143025 |
KSUAP-DM mReissners Membrane I24hrB |
| GSM143026 |
KSUAP-DM mReissners Membrane I24hrC |
| GSM143027 |
KSUAP-DM mReissners Membrane F0hrA |
| GSM143028 |
KSUAP-DM mReissners Membrane F0hrB |
| GSM143029 |
KSUAP-DM mReissners Membrane F0hrC |
|
| Relations |
| BioProject |
PRJNA100681 |
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