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Series GSE62013 Query DataSets for GSE62013
Status Public on Oct 03, 2014
Title Diabetes and Insulin in Regulation of Brain Cholesterol Metabolism
Organism Mus musculus
Experiment type Expression profiling by array
Summary The brain is the most cholesterol-rich organ in the body, most of which comes from in situ synthesis. Here we demonstrate that in insulin-deficient diabetic mice, there is a reduction in expression of the major transcriptional regulator of cholesterol metabolism, SREBP-2, and its downstream genes in the hypothalamus and other areas of the brain, leading to a reduction in brain cholesterol synthesis and synaptosomal cholesterol content. These changes are due, at least in part, to direct effects of insulin to regulate these genes in neurons and glial cells and can be corrected by intracerebroventricular injections of insulin. Knockdown of SREBP-2 in cultured neurons causes a decrease in markers of synapse formation and reduction of SREBP-2 in the hypothalamus of mice using shRNA results in increased feeding and weight gain. Thus, insulin and diabetes can alter brain cholesterol metabolism, and this may play an important role in the neurologic and metabolic dysfunction observed in diabetes and other disease states.
Overall design Hypothalamus was compared between streptozotocin (STZ)-induced diabetic, ob/ob, and control mice, with 5-6 replicates per goup.
Contributor(s) Suzuki R, Lee K, Jing E, Biddinger SB, McDonald JG, Montine TJ, Craft S, Kahn CR
Citation(s) 21109190
Submission date Oct 02, 2014
Last update date May 04, 2018
Contact name Jonathan M Dreyfuss
Organization name Joslin Diabetes Center
Street address 1 Joslin Pl
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (17)
GSM1518411 Control hypothalamus, rep1
GSM1518412 Control hypothalamus, rep2
GSM1518413 Control hypothalamus, rep3
BioProject PRJNA262937

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Supplementary file Size Download File type/resource
GSE62013_RAW.tar 39.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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