|
| Status |
Public on Oct 31, 2016 |
| Title |
Rb1+/+ versus Rb1ΔS/ΔS RNA Seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Upon G1-S transition, cyclin-dependent kinases (CDKs) phosphorylate the retinoblastoma tumor suppressor protein (pRB) to release E2F transcription factors, which activate transcriptional programs, required for S-phase entry. Beyond the G1-S transition, pRB activity remains poorly understood. Our lab has discovered that hyperphosphorylated pRB (ppRB), found beyond G1, retains exclusive binding to E2F1 through an alternate E2F1-‘specific’ binding site at the pRB c-terminus. We have developed a gene-targeted mouse model that is defective for the E2F1-‘specific’ interaction. We are exploring the function of this complex through genome-wide expression profiling. Overall, this work suggests an alternate pRB-E2F1 complex persists beyond the G1-S transition to establish regions of constitutive heterochromatin.
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| Overall design |
1. Total RNA from passage 4 quiescent MEFs isolated using TRIzol RNA extraction protocol
2. rRNA was depleted from total RNA using the RiboMinus Euk System V2 protocol according to manufacturer’s procotol
3. rRNA-depleted RNA samples were submitted for picoanalyzer analysis to determine concentration, purity, and rRNA content
4. Three wild-type and three mutant RNA samples with <10% rRNA remaining were submitted for library construction
5. Library was used for Illumina HiSeq 2500 paired end sequencing.
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| |
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| Contributor(s) |
Dick FA, Ishak CA, Marshall AE, Ferwati S |
| Citation(s) |
27889452 |
| Submission date |
Oct 03, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Fred Dick |
| E-mail(s) |
fdick@uwo.ca
|
| Organization name |
Western University
|
| Street address |
Cancer Research Labs 790 Commissioners Road East
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE85640 |
A commonly inactivated tumor suppressor silences endogenous retroelements in somatic cells |
|
| Relations |
| BioProject |
PRJNA263014 |
| SRA |
SRP048623 |
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