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| Status |
Public on Oct 07, 2014 |
| Title |
Follicular helper T cell signature in Type 1 Diabetes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under the control of the rat insulin promoter and co-expression of the DO11.10 transgene (DO11 x rip-mOVA mice). The transcriptome of T cells isolated from the pancreatic lymph nodes (lymph nodes draining the site of self antigen expression) was compared with that of T cells isolated from inguinal lymph nodes (non-draining lymph nodes). T cells were sorted based on expression of CD4, DO11.10 TCR (KJ-126), CD25 and CD69.
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| Overall design |
Primary cells from 6 week old DO11 x rip-mOVA mice were isolated ex-vivo from the pancreatic lymph nodes or inguinal lymph nodes. Cells were sorted by flow cytometry using antibodies to CD4, DO11.10 TCR (KJ-126+), CD25 and CD69. 3-6 replicates were collected per experimental group with each replicate deriving from 14 mice. RNA was isolated using the RNeasy micro kit (Qiagen).
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| Contributor(s) |
Walker L, Kenefeck R, Ono M |
| Citation(s) |
25485678 |
| Submission date |
Oct 06, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Lucy Walker |
| E-mail(s) |
lucy.walker@ucl.ac.uk
|
| Phone |
+44 20 7794 0500
|
| Organization name |
University College London
|
| Department |
Institute of Immunity & Transplantation
|
| Street address |
Royal Free Campus
|
| City |
London |
| ZIP/Postal code |
NW3 2PF |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA263199 |
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