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| Status |
Public on Oct 08, 2014 |
| Title |
Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells (methylation) |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Mastermind-like 1 (MAML1) is a transcriptional coregulator that has been associated with early development of many systems such as neuronal, muscular, cardiovascular and urogenital. The present study aimed to explore the genome-wide effects of MAML1 on gene expression and DNA methylation in human embryonic kidney cells. RNA expression was measured using a microarray that screens approximately 36,000 transcripts, and DNA methylation was determined for 450,000 CpG sites. 225 genes were found to be differentially expressed, while 11802 CpG sites were found to be differentially methylated in MAML1-expressing cells. A subset of 211 differentially methylated loci was associated with the expression of 85 genes. Gene ontology analysis revealed that these genes are involved in the regulation of urogenital system development, cell adhesion and embryogenesis.
The aim of the study was to identify genes regulated by mastermind-like pritein 1 and DNA methylation, using genome-wide approach.
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| Overall design |
We assayed the effects of MAML1 overexpression, as well as knock-down, on global DNA methylation profiles in HEK293 cells. 11802 CpG sites were found to be differentially methylated in MAML1-overexpressing cells. However, no significant changes were observed by comparing HEK293_H_siControl with HEK293_H_siMAML1.
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| Contributor(s) |
Putnik M, Brodin D, Fagerström-Billai F, Dahlman-Wright K, Wallberg AE |
| Citation(s) |
26857655 |
| Submission date |
Oct 08, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Milica Putnik |
| Organization name |
Karolinska Institute
|
| Department |
Biosciences and Nutrition
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| Street address |
Hälsovägen 7
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| City |
Huddinge |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE62178 |
Mastermind-like protein 1 regulates DNA methylation and expression of early developmental gene clusters in human embryonic kidney cells |
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| Relations |
| BioProject |
PRJNA263374 |
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