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| Status |
Public on Oct 14, 2014 |
| Title |
Cellular retinoic acid binding protein 2 (CRABP2) inhibits tumor growth by two distinct mechanisms |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
CRABP2 potently suppresses carcinoma cell growth, yet the mechanism(s) that underlie this activity remain incompletely understood. Two distinct functions are known for CRABP2: 1) the classical function of this protein is to directly deliver retinoic acid (RA) to the nuclear retinoic-acid receptorthereby activate gene expression, and 2) in the absence of RA, CRABP2 directly binds to the RNA-binding and stabilizing protein, HuR, and markedly strengthens its interactions with target mRNAs.
We used microarray experiments to elucidated genes regulated by HuR and/or CRABP2 in the absence of retinoic acid.
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| Overall design |
Two experiments were preformed: 1) Transcriptome profiles of MCF-7 cells overexpresssing shHuR were compared to control cells, both in the absence of retinoic acid. 2) Transcriptome profiles of MCF-7 cells overexpresssing shCRABP2 were compared to control cells, both in the absence of retinoic acid.
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| |
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| Contributor(s) |
Vreeland AC, Levi L, Zhang W, Berry DC, Noy N |
| Citation(s) |
25320093 |
| Submission date |
Oct 14, 2014 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Liraz Levi |
| E-mail(s) |
levil@ccf.org
|
| Organization name |
Lerner Research Institute; Cleveland Clinic
|
| Department |
Cellular and Molecular Medicine
|
| Lab |
Noa Noy
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA263790 |
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