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Series GSE6236 Query DataSets for GSE6236
Status Public on Mar 26, 2007
Title The human reticulocyte transcriptome
Organism Homo sapiens
Experiment type Expression profiling by array
Summary RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133 arrays were hybridized with probe from total RNA isolated from blood sampled from 14 umbilical cords and 14 healthy adult humans.
Keywords: Adult vs. fetal reticulocyte transcriptome comparison
 
Overall design Peripheral blood from 14 normal adult and 14 human umbilical cord were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133 A and B arrays.
 
Contributor(s) Goh S, Josleyn M, Lee YT, Danner RL, Gherman RB, Cam MC, Miller JL
Citation(s) 17405831
Submission date Nov 07, 2006
Last update date Aug 10, 2018
Contact name Jeffery Lynn Miller
E-mail(s) jm7f@nih.gov
Phone 301-480-1908
Fax 301-435-5148
Organization name NIH/NIDDK
Department Molecular Medicine Branch
Lab Molecular Biology and Genetics Section
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
Samples (56)
GSM143572 Adult_human_reticulocyte_transcriptome_AB01_HG-U133A
GSM143573 Adult_human_reticulocyte_transcriptome_AB02_HG-U133A
GSM143574 Adult_human_reticulocyte_transcriptome_AB03_HG-U133A
Relations
BioProject PRJNA97605

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Supplementary file Size Download File type/resource
GSE6236_RAW.tar 180.8 Mb (http)(custom) TAR (of CEL, EXP)

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