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Series GSE62373

Query DataSets for GSE62373

Status

Public on Jan 08, 2015

Title

Age-associated Changes in Basal NF-κB Function in Human CD4+ T Lymphocytes via Dysregulation of PI3 Kinase (dataset 3)

Organism

Experiment type

Expression profiling by array

Summary

Immune impairment and high circulating level of pro-inflammatory cytokines are landmarks of human aging. However, the molecular basis of immune dysregulation and the source of inflammatory markers remain unclear. Here we demonstrate that in the absence of overt cell stimulation, gene expression mediated by the transcription factor NF-κB is higher in purified and rested human CD4+ T lymphocytes from older compared to younger individuals. This increase of NF-κB -associated transcription includes transcripts for pro-inflammatory cytokines such as IL-1 and chemokines such as CCL2 and CXCL10. We demonstrate that NF-κB up-regulation is cell-intrinsic and mediated in part by phosphatidylinositol 3-kinase (PI3K) activity induced in response to metabolic activity, which can be moderated by rapamycin treatment. Our observations provide direct evidence that dysregulated basal NF-κB activity may contribute to the mild pro-inflammatory state of aging.

Overall design

Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood using Ficoll-Paque Plus density gradient centrifugation. CD4+ T cells were obtained by positive selection using anti-human CD4 microbeads. After incubation for 4hr at either 4 or 37 Degrees C, cells were harvested and collected by centrifugation. Total cellular RNA was prepared using Qiagen RNeasy protocols. Labeling and amplification were done with the standard Illumina protocol and the biotin-labeled cRNA was hybridized to Illumina's HumanHT-12 V3.0 Expression BeadChip. The arrays were washed, blocked and the biotin-labeled cRNA was detected by staining with streptavidin-Cy3.

Contributor(s)

Bektas A, Zhang Y, Lehrmann E, Wood III WH, Becker KG, Madara K, Ferrucci L, Sen R

Citation(s)

Submission date

Oct 15, 2014

Last update date

Jul 12, 2022

Contact name

Supriyo De

Organization name

NIA-IRP, NIH

Department

Laboratory of Genetics and Genomics

Lab

Computational Biology & Genomics Core

Street address

251 Bayview Blvd

City

Baltimore

State/province

Maryland

ZIP/Postal code

21224

Country

USA

Platforms (1)

Illumina HumanHT-12 V3.0 expression beadchip

Samples (45)

More...

GSM1526155	CD4+T cells_29 year Female_37ºC_Replicate-1
GSM1526156	CD4+T cells_30 year Male_37ºC_Replicate-1
GSM1526157	CD4+T cells_35 year Female_37ºC_Replicate-2

GSM1526158	CD4+T cells_40 year Male_37ºC_Replicate-2
GSM1526159	CD4+T cells_44 year Male_37ºC_Replicate-3
GSM1526160	CD4+T cells_44 year Male_37ºC_Replicate-4
GSM1526161	CD4+T cells_49 year Female_37ºC_Replicate-3
GSM1526162	CD4+T cells_52 year Male_37ºC_Replicate-5
GSM1526163	CD4+T cells_53 year Female_37ºC_Replicate-4
GSM1526164	CD4+T cells_54 year Male_37ºC_Replicate-6
GSM1526165	CD4+T cells_56 year Male_37ºC_Replicate-7
GSM1526166	CD4+T cells_61 year Male_37ºC_Replicate-8
GSM1526167	CD4+T cells_62 year Female_37ºC_Replicate-5
GSM1526168	CD4+T cells_63 year Male_37ºC_Replicate-9
GSM1526169	CD4+T cells_66 year Female_37ºC_Replicate-1
GSM1526170	CD4+T cells_67 year Male_37ºC_Replicate-1
GSM1526171	CD4+T cells_72 year Male_37ºC_Replicate-2
GSM1526172	CD4+T cells_73 year Female_37ºC_Replicate-2
GSM1526173	CD4+T cells_74 year Male_37ºC_Replicate-3
GSM1526174	CD4+T cells_76 year Male_37ºC_Replicate-4
GSM1526175	CD4+T cells_79 year Male_37ºC_Replicate-5
GSM1526176	CD4+T cells_81 year Female_37ºC_Replicate-3
GSM1526177	CD4+T cells_82 year Male_37ºC_Replicate-6
GSM1526178	CD4+T cells_29 year Female_4ºC_Replicate-1
GSM1526179	CD4+T cells_30 year Male_4ºC_Replicate-1
GSM1526180	CD4+T cells_35 year Female_4ºC_Replicate-2
GSM1526181	CD4+T cells_40 year Male_4ºC_Replicate-2
GSM1526182	CD4+T cells_44 year Male_4ºC_Replicate-3
GSM1526183	CD4+T cells_44 year Male_4ºC_Replicate-4
GSM1526184	CD4+T cells_49 year Female_4ºC_Replicate-3
GSM1526185	CD4+T cells_52 year Male_4ºC_Replicate-5
GSM1526186	CD4+T cells_53 year Female_4ºC_Replicate-4
GSM1526187	CD4+T cells_54 year Male_4ºC_Replicate-6
GSM1526188	CD4+T cells_56 year Male_4ºC_Replicate-7
GSM1526189	CD4+T cells_61 year Male_4ºC_Replicate-8
GSM1526190	CD4+T cells_62 year Female_4ºC_Replicate-5
GSM1526191	CD4+T cells_63 year Male_4ºC_Replicate-9
GSM1526192	CD4+T cells_67 year Male_4ºC_Replicate-10
GSM1526193	CD4+T cells_72 year Male_4ºC_Replicate-11
GSM1526194	CD4+T cells_73 year Female_4ºC_Replicate-6
GSM1526195	CD4+T cells_74 year Male_4ºC_Replicate-12
GSM1526196	CD4+T cells_76 year Male_4ºC_Replicate-13
GSM1526197	CD4+T cells_79 year Male_4ºC_Replicate-14
GSM1526198	CD4+T cells_81 year Female_4ºC_Replicate-7
GSM1526199	CD4+T cells_82 year Male_4ºC_Replicate-15

This SubSeries is part of SuperSeries:

Age-associated Changes in Basal NF-κB Function in Human CD4+ T Lymphocytes via Dysregulation of PI3 Kinase

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE62373_RAW.tar	6.2 Mb	(http)(custom)	TAR
GSE62373_non-normalized.txt.gz	8.6 Mb	(ftp)(http)	TXT
Processed data included within Sample table

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