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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 18, 2014 |
| Title |
Differential Roles of ERα and ERβ in Normal and Neoplastic Development in the Mouse Mammary Gland |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The present experiments were performed to determine the roles of estrogen receptors α and β (ERα and ERβ) in normal and neoplastic development in the mouse mammary gland. In wild-type mice, in vivo administration of estradiol (E) + progesterone (P) stimulated mammary ductal growth and alveolar differentiation. Mammary glands from mice in which the ERβ gene has been deleted (βERKO mice) demonstrated normal ductal growth and differentiation in response to E + P. By contrast, mammary glands from mice in which the ERα gene has been deleted (αERKO mice) demonstrated only rudimentary ductal structures that did not differentiate in response to E + P. EGF demonstrates estrogen-like activity in the mammary glands of αERKO mice: treatment of αERKO mice with EGF + P (without E) supported normal mammary gland development, induced expression of progesterone receptor (PR), and increased levels of G- protein-coupled receptor (GPR30) protein. Mammary gland development in βERKO mice treated with EGF + P was comparable to that of wild-type mice receiving EGF + P; EGF had no statistically significant effects on the induction of PR or expression of GPR30 in mammary glands harvested from either wild-type mice or βERKO mice. In vitro exposure of mammary glands to 7,12-dimethylbenz[a]anthracene (DMBA) induced preneoplastic mammary alveolar lesions (MAL) in glands from wild-type mice and βERKO mice, but failed to induce MAL in mammary glands from αERKO mice. Microarray analysis of DMBA-treated mammary glands identified 28 functional pathways whose expression was significantly different in αERKO mice versus both βERKO and wild-type mice; key functions that were differentially expressed in αERKO mice included cell division, cell proliferation, and apoptosis. The data demonstrate distinct roles for ERα and ERβ in normal and neoplastic development in the mouse mammary gland, and suggest that EGF can mimic the ERα-mediated effects of E in this organ.
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| Overall design |
Gene expression of mammary gland organ culture and DMBA-induced lesions from 4 mouse strains.
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| Contributor(s) |
Mehta RG, Hawthorne M, Mehta RR, Torres KE, Peng X, McCormick DL, Kopelovich L |
| Citation(s) |
25405629 |
| Submission date |
Oct 17, 2014 |
| Last update date |
Jan 26, 2015 |
| Contact name |
Michael Falduto |
| E-mail(s) |
mfalduto@genusbiosystems.com
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| Phone |
847-291-9602
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| Organization name |
GenUs BioSystems, Inc.
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| Street address |
1808 Janke, Unit M
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| City |
Northbrook |
| State/province |
IL |
| ZIP/Postal code |
60062 |
| Country |
USA |
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| Platforms (1) |
| GPL8063 |
GE Healthcare/Amersham Biosciences CodeLink™ Mouse Whole Genome Bioarray |
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| Samples (7)
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| GSM1527098 |
Mammary glands, ESR1 KO, 24day culture |
| GSM1527099 |
Mammary glands, ESR2 KO, 24day culture |
| GSM1527100 |
Mammary glands, C57 mice, 24day culture + DMBA |
| GSM1527101 |
Mammary glands, C57 mice, 10day culture, no DMBA |
| GSM1527102 |
Mammary glands, C57 mice, 24day culture, no DMBA |
| GSM1527103 |
Mammary glands, Balb/c mice, 24day culture |
| GSM1527104 |
Mammary glands, Balb/c mice, 24day culture + Vitamin D5 |
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| Relations |
| BioProject |
PRJNA264148 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62451_RAW.tar |
9.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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