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Series GSE62517 Query DataSets for GSE62517
Status Public on Mar 30, 2015
Title Flow-induced Protein Kinase A / CREB pathway acts via BMP signaling to promote AGM hematopoiesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary By chemical modulation of the PKA/CREB and BMP pathways in isolated AGM VE-cadherin+ cells from mid-gestation embryos, we demonstrate that PKA/CREB regulates hematopoietic engraftment and clonogenicity of hematopoietic progenitors and is dependent on secreted BMP ligands through the type I BMP receptor.
We used microarray to document upregulation of PKA/CREB-BMP pathway as well as global BMP target upregulation upon PKA/CREB activation.
 
Overall design Isolated VE+ cells from E11.5 AGM were treated with BMP4 (4ng/ml), forskolin (25uM) or both for 8 hours before RNA isolation.
 
Contributor(s) Kim PG, Daley GQ
Citation(s) 25870201
Submission date Oct 20, 2014
Last update date Feb 11, 2019
Contact name Peter Geon Kim
Organization name Dana Farber Cancer Institute
Department Hematology/Oncology
Lab Ben Ebert
Street address 360 Longwood Ave, Lc8202
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM1528126 DMSO,biological replicate 1
GSM1528127 Forskolin,biological replicate 1
GSM1528128 BMP4,biological replicate 1
Relations
BioProject PRJNA264319

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62517_RAW.tar 31.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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