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Series GSE62710 Query DataSets for GSE62710
Status Public on May 25, 2015
Title Commensal microbiota constantly control maturation and function of microglia in the central nervous system
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary As tissue macrophages of the central nervous system (CNS), microglia are critically involved in diseases of the CNS. However, it remains unknown what controls their maturation and activation under homeostatic conditions. Here we reveal significant contributions of the host microbiota to microglia homeostasis as germ-free (GF) mice displayed global defects in microglia with altered cell proportions and an immature phenotype leading to impaired innate immune responses. Temporal eradication of host microbiota severely changed microglia properties. Limited microbiota complexity also resulted in defective microglia. In contrast, recolonization with a complex microbiota partially restored microglia features. We determined that short-chain fatty acids (SCFA), microbiota-derived bacterial fermentation products, regulate microglia homeostasis. Accordingly, mice deficient for the SCFA receptor FFAR2 mirrored microglia defects found under GF conditions. These findings reveal that host bacteria vitally regulate microglia maturation and function, whereas microglia impairment can be restored to some extent by complex microbiota.
 
Overall design Examination of 1000 microglia cells per brain (SPF conditions: 7 samples and germ-free conditions: 7 samples)
 
Contributor(s) Erny D, Hrabě de Angelis AL, Jaitin DA, Wieghofer P, Staszewski O, David E, Keren-Shaul H, Mahlakoiv T, Jacobshagen K, Buch T, Schwierzeck V, Utermöhlen O, Chun E, Garrett WS, McCoy KD, Diefenbach A, Staeheli P, Stecher B, Amit I, Prinz M
Citation(s) 26030851
Submission date Oct 24, 2014
Last update date May 15, 2019
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (14)
GSM1531898 SPF1
GSM1531899 SPF2
GSM1531900 SPF3
Relations
BioProject PRJNA264845
SRA SRP049261

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE62710_Erny_RNAseq_data.xlsx 3.3 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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