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| Status |
Public on Sep 06, 2016 |
| Title |
Gene expression induced in HEK293 by treatment with the differentiating agents all-trans retinoic acid (tRA) and N6,2'-O-dibutyryl-cAMP (cAMP) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This gene array analysis shows that the transcriptome of HEK293, representing low-differentiated human embryonic kidney cells, cultured in presence of tRA and cAMP differentiating agents, is consistent with a genetic program relevant to kidney development
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| Overall design |
Comparison of HEK293 cells grown in standard contitions or in presence of differentiating agents. Cells were seeded in 10 cm dishes, cultured for 3 days either in complete medium or in medium supplemented with tRA and cAMP, harvested, and spun down before RNA isolation. Comparative analysis allowed us to identify genes transcriptionally by tRA/cAMP.
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| |
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| Contributor(s) |
De Cecco L, Fiorino A |
| Citation(s) |
27425396 |
| Submission date |
Oct 27, 2014 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
|
| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA264953 |
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