 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 02, 2015 |
| Title |
The DREAM complex promotes gene body deposition of H2A.Z for target repression |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
As part of a study of the function of the C. elegans DREAM complex, here we have used ChIP-seq to analyze the patterns of histone variant HTZ-1/H2A.Z and various histone modifications in lin-35(n745) mutants. In addition, we have used RNA-seq to analyze gene expression in lin-35 and htz-1 mutants as compared with wild-type L3 larvae.
|
| |
|
| Overall design |
EXPERIMENT TYPE: CHIP-seq, RNA-seq. BIOLOGICAL SOURCE: Strain: N2, JA1507, VC2490; Developmental Stage: L3 Larva; Genotype: wild type, lin-35(n745), htz-1(ok3099); Sex: mixed Male and Hermaphrodite population; EXPERIMENTAL FACTORS: Developmental Stage L3 Larva; temp (temperature) 20 degree celsius; Antibodies: HTZ-1 JA00001, H3K4me3 Wako 305-34819, H4ac4 Active Motif 39177, Histone H3 Abcam ab1791
|
| |
|
| Contributor(s) |
Ahringer J, Chesney M, Latorre I, Appert A, Kolasinska-Zwierz P, Stempor P |
| Citation(s) |
25737279 |
| Submission date |
Oct 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Przemyslaw Aleksander Stempor |
| Organization name |
University of Cambridge
|
| Department |
The Gurdon Institute
|
| Lab |
Ahringer Lab
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| State/province |
United Kingdom |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
| GPL13776 |
Illumina Genome Analyzer IIx (Caenorhabditis elegans) |
|
| Samples (20)
|
| GSM1534070 |
ChIP-seq of H3K36me3 in wild-type, replicate 1 |
| GSM1534071 |
ChIP-seq of H3K36me3 in wild-type, replicate 2 |
| GSM1534072 |
ChIP-seq of histone H3 in wild-type, replicate 1 |
| GSM1534073 |
ChIP-seq of histone H3 in wild-type, replicate 2 |
| GSM1534074 |
ChIP-seq of histone H3 in lin-35 mutant, replicate 1 |
| GSM1534075 |
ChIP-seq of histone H3 in lin-35 mutant, replicate 2 |
| GSM1534076 |
ChiP-seq of HTZ-1 in lin-35 mutant, replicate 1 |
| GSM1534077 |
ChiP-seq of HTZ-1 in lin-35 mutant, replicate 2 |
| GSM1534078 |
ChiP-seq of H3K4me3 in lin-35 mutant, replicate 1 |
| GSM1534079 |
ChIP-seq of H3K4me3 in lin-35 mutant, replicate 2 |
| GSM1534080 |
ChIP-seq of H4ac (tetraacetyl) in lin-35 mutant, replicate 1 |
| GSM1534081 |
ChIP-seq of H4ac (tetraacetyl) in lin-35 mutant, replicate 2 |
| GSM1534082 |
Input DNA for ChIPs, lin-35 mutant extract IL3 |
| GSM1534083 |
Input DNA for ChIPs, lin-35 mutant extract IL5 |
| GSM1534084 |
RNA-seq in wild-type, replicate 1 |
| GSM1534085 |
RNA-seq in wild-type, replicate 2 |
| GSM1534086 |
RNA-seq in lin-35 mutants, replicate 1 |
| GSM1534087 |
RNA-seq in lin-35 mutants, replicate 2 |
| GSM1534088 |
RNA-seq in htz-1 mutants, replicate 1 |
| GSM1534089 |
RNA-seq in htz-1 mutants, replicate 2 |
|
| Relations |
| BioProject |
PRJNA265795 |
| SRA |
SRP049395 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62833_N2_lin-35_htz-1_processed_RNAseq.xlsx |
3.3 Mb |
(ftp)(http) |
XLSX |
| GSE62833_RAW.tar |
3.8 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...