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| Status |
Public on Nov 18, 2006 |
| Title |
Transcriptional profile of E14.5 micro-dissected kidney structures: renal vesicle, s-shaped body & collecting duct (MOE430A platform). (GUDMAP Series ID: 8) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Our laboratory's interest is in understanding the molecular principles that underlie the regional organization of the mammalian metanephric kidney. Our goal is to generate a detailed spatial map of the cellular expression of selected regulatory genes during mammalian kidney development. The goal of this study is to identify genes expressed during the morphogenesis of the nephron. By profiling two specific developmental nephron structures, we expect to identify genes expressed in both, and unique to each, which suggests they may play a role in the underlying mechanism responsible for the morphogenesis.
Keywords: kidney component comparison
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| Overall design |
Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays. Detection of genes previously shown to be expressed in these early structures, such as Wt1, Wnt4 and Pax8, validates the data from the screen. The collecting duct sample serves as a reference sample for comparing the renal vesicle and s-shaped body comparison in this study.
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| Contributor(s) |
McMahon AP, Valerius MT |
| Citation missing |
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| Submission date |
Nov 15, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
gudmap-db@gudmap.org
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| Phone |
+44 131 651 8500
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| Organization name |
IGMM MRC Human Genetics Unit
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| Lab |
GUDMAP Database Group
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| Street address |
Crewe Road
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| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA100593 |
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