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Series GSE6298 Query DataSets for GSE6298
Status Public on May 01, 2008
Title Comparison of gene expression profile of human trabecular meshwork cells induced by triamcinolone with dexamethasone
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PURPOSE. Triamcinolone acetonide (TA) and dexamethasone (DEX) are corticosteroids commonly used for ocular inflammation but both can cause ocular hypertension. We investigated the differential gene expression profile of human trabecular meshwork (TM) cells in response to treatment by TA compared to that by DEX.
METHODS. Total RNA was extracted from cultured human TM cells treated with TA or DEX and used for microarray gene expression analysis. The microarray experiments were repeated three times. Differentially expressed genes were identified by an empirical Bayes approach and confirmed by real-time quantitative PCR.
RESULTS. 0.1 mg/ml TA treatment resulted in 15 genes up-regulated and 12 genes down-regulated while 1mg/ml TA treatment resulted in 36 genes up-regulated and 21 genes down-regulated. These genes were mainly associated with acute-phase response, cell adhesion, cell cycle and growth, growth factor, ion binding, metabolism, proteolysis and transcription factor. Two genes, MYOC and GAS1, were up-regulated and 3 genes, SENP1, ZNF343 and SOX30, were down-regulated by both TA and DEX treatment. Eight differentially expressed genes were located in known primary open angle glaucoma (POAG) loci, including MYOC, SOAT1, CYP27A1, SPOCK, SEMA6A, EGR1, GAS1 and ATP10A.
CONCLUSIONS. Differential gene expression profiles of human TM cells treated by TA and DEX, and a dosage effect by TA, were revealed by microarray technology. TA and DEX treatment shared several differentially expressed genes, suggesting a common mechanism to cause ocular hypertension. Some differentially expressed genes located in the known POAG loci are potential candidates for glaucoma genes.
Keywords: Differential gene expression profile
Overall design A total of 9 samples were used for this study. There were 3 biological replicates for each of 3 treatments.
Contributor(s) Fan BJ, Wang DY, Tham CC, Lam SC, Pang CP
Citation(s) 18436822
Submission date Nov 16, 2006
Last update date Jan 18, 2013
Contact name Bao Jian Fan
Phone 617-573-6448
Fax 617-573-6439
Organization name Harvard Medical School
Department Ophthalmology
Lab Howe/Wiggs Lab
Street address MEEI, 243 Charles Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL4564 Stanford Human cDNA SHEW
Samples (9)
GSM144696 TM_TA_0.1mg/ml_rep1
GSM144701 TM_TA_0.1mg/ml_rep2
GSM144702 TM_TA_0.1mg/ml_rep3
BioProject PRJNA100615

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