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Series GSE62999 Query DataSets for GSE62999
Status Public on Nov 06, 2014
Title Expression data from study on IL33 signaling in Dusp5 deficient eosinophils
Organism Mus musculus
Experiment type Expression profiling by array
Summary Dusp5 regulates ERK phosporylation following IL-33 receptor ligation in cultured eosinophils. Dusp5 deficient eosinophils show increased ERK phosphorylation, and as a result are less apoptotic. Since ERK stimulation results in downstream activation of transcription factors, we are utilizing a microarray approach to find alterations in gene expression to uncover potential mechanisms for increased cell survival.
Overall design Day 12 bone marrow derived eosinophils from Dusp5+/+ and Dusp5-/- mice (5 mice per genotype) were left untreated (media only) or IL-33 (25ng/ml) stimulated for 4 hours. Treated cells were washed with ice cold PBS and cell pellets were directly lysed in RLT buffer (Qiagen) followed by immediate RNA isolation (RNEasy plus mini kit with genomic DNA eliminator columns). Sample group names: Group 1- Dusp5+/+ Untreated (N=5) Group 2- Dusp5-/- Untreated (N=5) Group 3- Dusp5+/+ IL-33 4hrs (N=5) Group 4- Dusp5-/- IL-33 4hrs (N=5) Sample Names: Group 1- BM Eos unstimulated WT-1....WT-5 Group 2- BM Eos unstimulated Dusp5-/- -1......Dusp5-/- -5 Group 3- BM Eos IL-33 4hrs WT-1....WT-5 Group 4- BM Eos IL-33 4hrs Dusp5-/- -1......Dusp5-/- -5
Contributor(s) Holmes DA, Yeh J, Yan D, Xu M, Modrusan Z, Chan AC
Citation(s) 25398911
Submission date Nov 05, 2014
Last update date Feb 11, 2019
Contact name Derek A Holmes
Phone +1 650 467 0939
Organization name Genentech Inc
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (20)
GSM1537848 Bonemarrow_WT_Untreated_rep1
GSM1537849 Bonemarrow_WT_Untreated_rep2
GSM1537850 Bonemarrow_WT_Untreated_rep3
BioProject PRJNA266408

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Supplementary file Size Download File type/resource
GSE62999_RAW.tar 64.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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