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| Status |
Public on Nov 08, 2014 |
| Title |
BMSC gene expression in co-culture with endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this experiment human Bone Marrow Stem Cells (hBMSCs) are grown in co-culture with Human Umbilical Vein Endothelial Cells (HUVECs) for 5 days, HUVECs are removed by trypsination before call harvest. In parallel hBMSCs are grown in monoculture as a control for 5 days, and a sham trypsination performed before harvesting of the cells.
The aim for the project is to analyze the gene expression alteration in hBMSCs resulting from BMSCs being co-cultured with HUVECs.
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| Overall design |
The microarray platform chosen is Illumina, which is a one-channel system, and this experiment uses HumanWG-6 v3.0 Expression BeadChips. The 1-channel nature of the platform yields the simple design of 1 sample hybridized to 1 array on a microarray slide. This particular chip has 6 arrays on each slide. At each time points there were 6 samples in the co-culture (T) group and 6 samples in the control (C) group, all together 12 samples. Samples came from 6 different donors denoted D1-D6, and the sample from donor X in the co-culture group is paired with the sample from donor X in the control group.
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| Contributor(s) |
Xue Y, Mustafa K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 07, 2014 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Ying Xue |
| E-mail(s) |
ying.xue@uib.no
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| Phone |
+47 96676035
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| Organization name |
University of Bergen
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| Department |
Department of Dentistry
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| Street address |
Årstadveien 19
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| City |
Bergen |
| ZIP/Postal code |
5009 |
| Country |
Norway |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA266619 |
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