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Series GSE6327 Query DataSets for GSE6327
Status Public on Aug 01, 2007
Title Histone H3 K4,36,79R 6 hr
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Total RNA from two replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H3 K4,36,79R mutant. Cells were grown in galactose media and then shifted to glucose media for 6 hours to give the 6 hour time point.
Keywords: repeat
 
Overall design Histone H3 K4,36,79R time course. Cells were grown in galactose media and then shifted to glucose media, which repressed the expression of wild-type histone H3, leaving the histone H3 K4,36,79R mutant as the sole source of histone H3 protein in the cell. Total RNA was isolated from replicate cultures of wild-type and mutant histone H3 at various time points after the shift to glucose media (0, 6, and 9 hours). Expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H3 K4,36,79R mutant.
 
Contributor(s) Rodriguez AM, Jin Y, Wyrick JJ
Citation(s) 17664279
Submission date Nov 20, 2006
Last update date Jul 01, 2016
Contact name John J. Wyrick
E-mail(s) jwyrick@vetmed.wsu.edu
Phone 509-335-8785
Organization name Washington State University
Department School of Molecular Biosciences
Street address Biotechnology Life Sciences 241
City Pullman
State/province WA
ZIP/Postal code 99164
Country USA
 
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (6)
GSM146320 Wild-type for H3 K4,36,79R 6 hr replicate 1
GSM146321 Wild-type for H3 K4,36,79R 6 hr replicate 2
GSM146322 Wild-type for H3 K4,36,79R 6 hr replicate 3
This SubSeries is part of SuperSeries:
GSE6331 Histone H3,K4,79R and H3,K4,36,79R (at 0, 6, and 9 hours) Mutations
Relations
BioProject PRJNA105009

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6327_RAW.tar 8.3 Mb (http)(custom) TAR (of CEL)

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