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| Status |
Public on Sep 11, 2015 |
| Title |
Intra-amniotic Balloon Inflation Induces an Inflammatory “Pulse” and Labor in Nonhuman Primates |
| Platform organism |
Macaca mulatta |
| Sample organism |
Macaca nemestrina |
| Experiment type |
Expression profiling by array
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| Summary |
Uterine stretch is thought to induce preterm labor in women with twin and higher order pregnancies, but the pathophysiology remains unclear. We investigated the pathogenesis of stretch-induced preterm birth for the first time in a pregnant nonhuman primate model.
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| Overall design |
Eleven chronically catheterized pregnant monkeys (Macaca nemestrina) at 118-125 days gestation (term=172 days) received either: 1) inflation of intra-amniotic balloons (N=6) or 2) saline inoculation (N=5). Cesarean section and fetal necropsy was performed due to preterm labor or to collect tissues, except in one case where the animal delivered spontaneously, reducing samples for microarray analysis to ten (five stretch and five control animals). Amniotic fluid and maternal plasma were analyzed for multiple cytokines and prostaglandins using Luminex, enzyme-linked immunosorbent assay and Analysis of Covariance. Ribonucleic acid was extracted from the myometrium in the lower uterus at Cesarean section and analyzed by microarray and quantitative reverse transcriptase polymerase chain reaction.
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| Contributor(s) |
Adams Waldorf KM, Mohan AR, Young RC, Ngo L, Das A, Tsai J, Bansal A, Paolella L, Soorana SR, Gough M, Astley C, Vogel K, Baldessari AE, Bammler TK, MacDonald JW, Gravett MG, Rajagopal L, Johnson M |
| Citation(s) |
26284599 |
| Submission date |
Nov 13, 2014 |
| Last update date |
Dec 14, 2015 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
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| Organization name |
University of Washington
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| Department |
Environmental and Occupational Health Sciences
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| Street address |
4225 Roosevelt Way NE
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
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| Platforms (1) |
| GPL3535 |
[Rhesus] Affymetrix Rhesus Macaque Genome Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA267264 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63274_RAW.tar |
44.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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