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| Status |
Public on Nov 08, 2016 |
| Title |
Gene expression profile of wt-, S473A- and S473D-KAP1-re-expression in KAP1-depleted MDA-MB-231 cell |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We study the gene regulation function of serine 473 phosphorylation of KAP1 (pS473-KAP1) in MDA-MB-231 cells. Wild type KAP1, S473A-KAP1 (phospho-acceptor site mutant) and S473D-KAP1 (phospho-mimetic mutant) are re-expressed in KAP1 knockdown cells. We analyze the gene expression profile in these three cells and find that many mitochondrial complex genes are up-regulated in S473D-KAP1 re-expressing cells. This study provides information about pS473-KAP1-regulated gene expression.
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| Overall design |
mRNA profile of wt-, S473A- and S473D-KAP1-expressing MDA-MB-231 cells were generated by deep sequencing
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| Contributor(s) |
Cheng C, Ann DK |
| Citation(s) |
27364555 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM119388 |
Analysis of Fis1 in Mitophagy in Mammals |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
David C Chan |
|
| Submission date |
Nov 17, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
David Kong Ann |
| E-mail(s) |
dann@coh.org
|
| Organization name |
City of Hope
|
| Street address |
1500 E. Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA267545 |
| SRA |
SRP049947 |
