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Series GSE63679 Query DataSets for GSE63679
Status Public on Aug 27, 2016
Title Mesenchymal tumors can derive from pericytes.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We demonstrated that deletion of the p53 tumor suppressor gene in NG2 expressing cells resulted in the development of bone and soft tissue sarcomas that closely resemble human tumors. To determine gene expression differences between NG2 expressing pericytes lacking p53 and sarcomas that arose from deletion of p53 in NG2 expressing cells, RNA sequencing analysis was implemented.
Overall design We isolated total RNA from NG2 expressing pericytes (2 samples), which were sorted from skeletal muscle tissues of NG2-Cre;p53flox/flox mice using an NG2 antibody. We also isolated total RNA from osteosarcomas (2 samples) and soft tissue sarcomas (2 samples), which were developed in NG2-Cre;p53flox/flox mice. Differentially expressed genes were analyzed using Illumina HiSeq 2000.
Contributor(s) Sato S, Alman B
Citation(s) 27425618
Submission date Nov 26, 2014
Last update date May 15, 2019
Contact name Benjamin Alman
Organization name The Hospital for Sick Children
Department Developmental and Stem Cell Biology
Lab Benjamin Alman Lab
Street address PGCRL 16th Floor, 686 Bay Street
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A4
Country Canada
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM1555110 NG2 expressing cells 1
GSM1555111 NG2 expressing cells 2
GSM1555112 Osteosarcoma 1
BioProject PRJNA268679
SRA SRP050308

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63679_RAW.tar 910.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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