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Series GSE63735 Query DataSets for GSE63735
Status Public on Nov 25, 2015
Title PRMT6 knockdown effects on the early zebrafish development
Organism Danio rerio
Experiment type Expression profiling by array
Summary Zygotic genome activation (ZGA), which is according to the midblastula transition in zebrafish, is an important event during the maternal-zygotic transition in animals. Our preliminary study and other group’s works indicate that epigenetic regulations play an essential role in ZGA.
Morpholino was employed to knockdown PRMT6. We used microarrays to analyze the global gene expression in prmt6 morphants.
Overall design prmt6 MO (0.3mM) was injected into the one-two cell zebrafish, prmt6 cMO (0.3mM) injection as a control. At 6 hpf, embryos were classified into three subtypes (normal, mild and severe) and prepared for global gene expression analysis with Affymetrix Zebrafish Genome Arrays. The severe subtype and the control were repeated three times.
Contributor(s) Zhao X, Li Y
Citation(s) 26487724
Submission date Dec 01, 2014
Last update date Jan 25, 2018
Contact name xin xi zhao
Organization name Shanghai Institute of Biochemistry and Cell Biology
Street address Yueyang Road, 320#, new building 615
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (8)
GSM1556311 embryo of cMO injection, biological rep 1
GSM1556312 embryo of cMO injection, biological rep 2
GSM1556313 embryo of cMO injection, biological rep 3
BioProject PRJNA269151

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Supplementary file Size Download File type/resource
GSE63735_RAW.tar 14.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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