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| Status |
Public on Dec 13, 2014 |
| Title |
Genome-Wide Mapping of 5-mC and 5-hmC in severely Preeclamptic Placentas. |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Preeclampsia (PE) is a major contributor of maternal mortality with uncertain etiology. Recent studies suggested that epigenetic modifications, including DNA methylation, play a vital role in the development of PE. In this study, we have mapped genome-wide distribution of 5-methylcytosin (5-mC) and 5-hydroxymethylcytosine (5-hmC) using MeDIP and (h)MeDIP in the placentas from severely preeclamptic patients and normal controls. A total 194485 pooled 5-mC peaks and 138133 pooled 5-hmC peaks were identified, of which 714 5-mC peaks and 119 5-hmC peaks showed significant difference between patients and controls (>2-fold, p<0.05).To our knowledge, this is the first report of DHMRs (Differentially Hydroxy-Methylated Regions) in preeclamptic placenta. We not only confirmed the aberrant DNA methylated regions in the process of preeclampsia reported previously, but also identified unreported regions. A total of 4 selected DMRs (Differentially Methylated Regions) were also confirmed by MassARRAY EppiTYPER. Of these, PTPRN2, which had low level of 5-mC and high level of 5-hmC at gene body, was further verified to have lower methylation level at promoter regions in case group compared with controls. In conclusion, our study provided genome-wide distribution of 5-mC and 5-hmC in severe PE and normal controls, which have further clinical value for the identification of diagnostic and therapeutic markers for severe PE.
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| Overall design |
Examination of 5-mC and 5-hmC pattern in 4 control cases' tissue and 4 severely Preeclamptic Placentas cases' tissue.
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| Contributor(s) |
Xiaotian L, Fei L, Lisha Z, Ruitu L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 01, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Li Xiaotian |
| E-mail(s) |
xiaotianli555@163.com
|
| Phone |
18521304014
|
| Organization name |
Obstetrics and Gynecology Hospital of Fudan University
|
| Department |
Obstetrics Department
|
| Lab |
Obstetrics Department
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| Street address |
Fangxie Road 419 in Huangpu District
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA268963 |
| SRA |
SRP050385 |
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