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Series GSE6376 Query DataSets for GSE6376
Status Public on Jan 30, 2007
Title Zymosan stimulation of MyD88-deficient bone marrow-derived macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Innate immune pattern recognition receptors play critical roles in pathogen detection and initiation of antimicrobial responses. We and others have previously demonstrated the importance of the beta-glucan receptor Dectin-1 in the recognition of pathogenic fungi by macrophages and dendritic cells, and have elucidated some of the mechanisms by which Dectin-1 signals to coordinate the antifungal response. While Dectin-1 signals alone are sufficient to trigger phagocytosis and Src-Syk-mediated induction of antimicrobial reactive oxygen species, collaboration with Toll-like receptor (TLR)2 signaling enhances NF-kB activation and regulates cytokine production. In this study we demonstrate that Dectin-1 signaling can also directly modulate gene expression via activation of nuclear transcription of activated T cells (NFAT) transcription factors. Dectin-1 ligation by zymosan particles or live Candida albicans yeast triggers NFAT activation in macrophages and dendritic cells. Dectin-1-triggered NFAT activation plays a role in the induction of Egr2 and Egr3 transcription factors, and cyclooxygenase 2 (Cox-2). Furthermore, we show that NFAT activation regulates IL-2, IL-10 and IL-12 p70 production by zymosan-stimulated dendritic cells. These data establish NFAT activation in myeloid cells as a novel mechanism of regulation of the innate antimicrobial response.
Keywords: stress response
Overall design Bone marrow-derived macrophages deficient in MyD88 were stimulated with zymosan, and total RNA was extracted 120 minutes after stimulation for comparison to macrophages grown under the same conditions, but not stimulated.
Contributor(s) Goodridge HS, Simmons RS, Underhill DM
Citation(s) 17312158
Submission date Nov 27, 2006
Last update date Aug 02, 2019
Contact name Bruz Marzolf
Phone 206-732-1352
Organization name Institute for Systems Biology
Street address
City Seattle
State/province WA
ZIP/Postal code 98103
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM147166 MyD88_LPS_M2-0
GSM147167 MyD88_PIC_C-0
GSM147168 MyD88_ZYM_A
BioProject PRJNA99601

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Supplementary file Size Download File type/resource
GSE6376_RAW.tar 14.1 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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