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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 05, 2014 |
| Title |
The Sm protein methyltransferase PRMT5, is not required for primordial germ cell specification in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
PRMT5 is a type II protein arginine methyltransferase with roles in stem cell biology, reprogramming, cancer and neurogenesis. During embryogenesis in the mouse it was hypothesized that PRMT5 functions with the master germline determinant BLIMP1 to promote primordial germ cell (PGC) specification. Using a Blimp1-Cre germline conditional knockout, we discovered that Prmt5 has no major role in murine germline specification, or the first global epigenetic reprogramming event involving depletion of cytosine methylation from DNA and histone H3 lysine 9 dimethylation from chromatin. Instead, we discovered that PRMT5 functions at the conclusion of PGC reprogramming I to promote proliferation, survival and expression of the gonadal germline program as marked by MVH. We show that PRMT5 regulates gene expression by promoting methylation of the Sm spliceosomal proteins, and significantly altering the spliced repertoire of RNAs in mammalian embryonic cells and primordial cells.
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| Overall design |
Examination of transcriptional profile of iPHet (Control) vs. iPKO (Prmt5 knock out) 2i Embryonic Stem Cells
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| Contributor(s) |
Clark AT, Li Z, Yu J |
| Citation(s) |
25519955 |
| BioProject |
PRJNA245758 |
| Submission date |
Dec 03, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Juehua Yu |
| E-mail(s) |
juehuayu@gmail.com
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| Organization name |
UCLA
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| Street address |
615 Charles E. Young South BSRB 457
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| SRA |
SRP041516 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE63800_RAW.tar |
8.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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