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| Status |
Public on Jun 17, 2015 |
| Title |
Comparison of the DNA methylation profiles of CD19+ B lymphocytes of a pair of monozygotic twins discordant for common variable immunodeficiency |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
The methylation profiles of bisulfite-modified DNA of CD19+ cells from MZ twins discordant for CVID were compared using the Infinium HumanMethylation450 BeadChips (Illumina, Inc., San Diego, CA,). This platform allows the interrogation of >485,000 methylation sites per sample at single-nucleotide resolution, and comprises an average of 17 CpG sites per gene in the 99% of RefSeq genes. 96% of CpG islands are covered, with additional coverage in CpG island shores and the regions flanking them. The samples were hybridized in the array following the manufacturer’s instructions.
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| Overall design |
Total DNA isolated by standard procedures from CD19+ cells (total B lymphocytes) corresponding to two monozygotic twins discordant for common variable immunodeficiency.
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| Contributor(s) |
Rodríguez-Cortez VC, del Pino-Molina L, Ciudad L, Gómez-Cabrero D, Company C, Tegnér J, Rodríguez-Gallego C, López-Granados E, Ballestar E |
| Citation(s) |
26081581 |
| Submission date |
Dec 04, 2014 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Esteban Ballestar |
| Organization name |
Josep Carreras Research Institute (IJC)
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| Lab |
Epigenetics and Immune Disease
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| Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
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| City |
Badalona, Barcelona |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (6)
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| GSM1558470 |
CD19+ cells control/healthy twin (replicate 1) |
| GSM1558471 |
CD19+ cells control/healthy twin (replicate 2) |
| GSM1558472 |
CD19+ cells control/healthy twin (replicate 3) |
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| Relations |
| BioProject |
PRJNA269256 |
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