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| Status |
Public on Nov 23, 2016 |
| Title |
RNA-seq profiling of Hoxa2/Hoxb2 mutants versus wild type in Math1 positive cells from the Cochlear Nucleus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Differential gene expression was analyzed for FACS sorted Math1::Cre; ROSA-tdTomato from hand dissected cochlear nuclei of wild type and Hoxa2/Hoxb2 mutant mice
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| Overall design |
In order to investigate the role of Hoxa2 and Hoxb2 transcription factors in a subset of cells of the cochlear nucleus, we generated double conditional knock-out by crossing the deleter line Math1::Cre crossed with Rosa tdTomato; Hoxa2fl/fl; Hoxb2fl/fl and Rosa tdTomato wild type background. FACS sorted cells from hand dissected cochlear nuclei were than processed and RNA-seq performed (see extract protocol and library construction protocol).
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| Contributor(s) |
Loche A, Karmakar K, Rijli F, Thiry S |
| Citation(s) |
28052248 |
| Submission date |
Dec 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Tim Roloff |
| E-mail(s) |
tim.roloff@fmi.ch
|
| Organization name |
FMI
|
| Department |
Functional Genomics
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| GSM1564183 |
Math1::Cre; Rosa tdTomato rep 4 |
| GSM1564184 |
Math1::Cre; Rosa tdTomato; Hoxa2+/fl; Hoxb2fl/fl rep 1 |
| GSM1564185 |
Math1::Cre; Rosa tdTomato; Hoxa2+/fl; Hoxb2fl/fl rep 2 |
| GSM1564186 |
Math1::Cre; Rosa tdTomato; Hoxa2+/fl; Hoxb2fl/fl rep 3 |
| GSM1564187 |
Math1::Cre; Rosa tdTomato; Hoxa2+/fl; Hoxb2fl/fl rep 4 |
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| Relations |
| BioProject |
PRJNA270100 |
| SRA |
SRP051077 |
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