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Series GSE64096 Query DataSets for GSE64096
Status Public on Jan 31, 2015
Title Intra-sample heterogeneity of sperm DNA methylation
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Genome wide DNA methylation profiling of 2 distinct subpopulations of sperm within a single ejaculate. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs. Samples were provided by 20 normozoospermic individuals. A density gradient centrifugation was performed on each samples to yeild two distict populations for each ejaculate (one from the 90% isolate layer high quality sperm and one from the 35% isolate layer low quality sperm of the column).
 
Overall design Bisulphite converted DNA from the 40 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip
 
Contributor(s) Jenkins TG, Carrell DT
Citation(s) 25542834
Submission date Dec 11, 2014
Last update date Mar 22, 2019
Contact name Douglas Carrell
Organization name University of Utah
Street address 675 Arapeen Dr. Suite 201
City Salt Lake City
State/province UT
ZIP/Postal code 84108
Country USA
 
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (40)
GSM1564241 sperm genomic DNA from Donor A (0.9 subpopulatoin)
GSM1564242 sperm genomic DNA from Donor A (0.35 subpopulatoin)
GSM1564243 sperm genomic DNA from Donor B (0.9 subpopulatoin)
Relations
BioProject PRJNA270108

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64096_RAW.tar 510.3 Mb (http)(custom) TAR (of IDAT)
GSE64096_unmethylated_methylated.txt.gz 165.2 Mb (ftp)(http) TXT
Processed data included within Sample table
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