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Series GSE6424 Query DataSets for GSE6424
Status Public on Dec 06, 2006
Title ß-catenin Plays a Dual Role in the Generation and Maintenance of Immortalized Hematopoietic Stem Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hematopoietic stem cells (HSCs) are characterized by their ability to give rise to all mature blood lineages and to renew themselves. Despite their potential for the therapeutic use in cell replacement therapy, the cell fate decisions of HSCs controlled by extrinsic and intrinsic mechanisms remain poorly understood. In summary, our results provide a number of novel and important observations illustrating and enlightening the importance of ß-catenin in hematopoiesis. Specifically, we show that ß-catenin plays an essential role in the immortalization of HSCs.
Keywords: hematopoietic stem cells , immortalization, ß-catenin, genetic modification, time course
 
Overall design One objective of our study was to understand the molecular targets of ß-catenin in controlling the immortalization of hematopoietic stem cells (HSCs). Using a microarray screening approach, we had identified candidate genes that might potentially regulate ß-catenin mediated immortalization of HSCs. A special focus was given to transcription factors (TFs), since they play myriad functions in basic cellular processes. The strategy of our experimental design utilized a combination of a pair wise comparison and time course microarray experiments that include the expression profiles of TFs in 4 different samples:
 
Contributor(s) Geffers R, Templin C, Kotlarz D
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Submission date Dec 04, 2006
Last update date Feb 11, 2019
Contact name Robert Geffers
E-mail(s) robert.geffers@helmholtz-hzi.de
Phone +49 531-6181-3058
Organization name HCI - Helmholtz Centre for Infection Research
Department Dep. Molecular Bacteriology
Lab Genome Analytics
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM148077 ß-catenin transduced LSK day 3
GSM148078 eGFP transduced LSK day 3
GSM148079 DKmix cells day 50
Relations
BioProject PRJNA99497

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6424_RAW.tar 13.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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