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Status |
Public on Mar 16, 2015 |
Title |
Identification of Tox chromatin binding properties and of its downstream targets by DamID |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Purpose: The goal of this study was to characterize the chromatin binding features and the transcriptional target genes of a new key player during corticogenesis, the transcription factor Tox Methods: DamID was performed using two cell lines, the Neuro-2a and the HEK-293T cells. Experiments were performed in duplicates for each cell line by infecting them with pLgwV5Eco-ToxDam lentiviruses and using the pLgxV5Dam viruses as control. DamID was performed as described in Vogel et al., 2006. Results: Our analysis identifyed ca. 13,000 Tox chromatin binding regions
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Overall design |
DamID was performed using two cell lines, the Neuro-2a and the HEK-293T cells. Experiments were performed in duplicates for each cell line by infecting them with pLgwV5Eco-ToxDam lentiviruses and using the pLgxV5Dam viruses as control. DamID was performed as described in Vogel et al., 2006.
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Contributor(s) |
Artegiani B, de Jesus Domingues AM, Dahl A, Calegari F |
Citation(s) |
25527292, 26981424 |
Submission date |
Dec 16, 2014 |
Last update date |
Jul 03, 2019 |
Contact name |
Federico Calegari |
E-mail(s) |
federico.calegari@tu-dresden.de
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Organization name |
TU Dresden
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Department |
Center for Molecular and Cellular Bioengineering (CMCB)
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Lab |
Calegari Lab
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Street address |
Fetscherstr. 105
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City |
Dresden |
ZIP/Postal code |
01307 |
Country |
Germany |
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Platforms (2) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (11)
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Relations |
BioProject |
PRJNA270582 |
SRA |
SRP051238 |