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Series GSE64240 Query DataSets for GSE64240
Status Public on Mar 16, 2015
Title Identification of Tox chromatin binding properties and of its downstream targets by DamID
Organisms Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: The goal of this study was to characterize the chromatin binding features and the transcriptional target genes of a new key player during corticogenesis, the transcription factor Tox
Methods: DamID was performed using two cell lines, the Neuro-2a and the HEK-293T cells. Experiments were performed in duplicates for each cell line by infecting them with pLgwV5Eco-ToxDam lentiviruses and using the pLgxV5Dam viruses as control. DamID was performed as described in Vogel et al., 2006.
Results: Our analysis identifyed ca. 13,000 Tox chromatin binding regions
 
Overall design DamID was performed using two cell lines, the Neuro-2a and the HEK-293T cells. Experiments were performed in duplicates for each cell line by infecting them with pLgwV5Eco-ToxDam lentiviruses and using the pLgxV5Dam viruses as control. DamID was performed as described in Vogel et al., 2006.
 
Contributor(s) Artegiani B, de Jesus Domingues AM, Dahl A, Calegari F
Citation(s) 25527292, 26981424
Submission date Dec 16, 2014
Last update date Jul 03, 2019
Contact name Federico Calegari
E-mail(s) federico.calegari@tu-dresden.de
Organization name TU Dresden
Department Center for Molecular and Cellular Bioengineering (CMCB)
Lab Calegari Lab
Street address Fetscherstr. 105
City Dresden
ZIP/Postal code 01307
Country Germany
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (11)
GSM1566841 ToxDam_Rep1_HEK293T
GSM1566842 ToxDam_Rep2_HEK293T
GSM1566843 Dam_Rep1_HEK293T
Relations
BioProject PRJNA270582
SRA SRP051238

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64240_human_cell_line_sicer_peaks_Tox-W50-G250-islands-summary-FDR0.01.txt.gz 2.2 Mb (ftp)(http) TXT
GSE64240_mouse_cell_line_sicer_peaks_Tox-W50-G250-islands-summary-FDR0.001.txt.gz 2.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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