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| Status |
Public on Jan 31, 2016 |
| Title |
A ChIP-seq spike-in method enables detection of global histone modification changes across the genome |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study outlines a method that dramatically alters the interpretation of ChIP-seq data and will improve the quantitative comparison of histone modification maps across biological contexts or across various conditions within a given biological context.
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| Overall design |
We introduced a small fraction of Drosophila chromatin into human ChIP samples and added a Drosophila-specific antibody as a means to consistently precipitate Drosophila chromatin as a minor fraction of the total ChIP DNA. The Drosophila ChIP-seq tags are used to normalize the human ChIP-seq data from DMSO and EZH2 inhibitor-treated samples. Employing this strategy, a substantial reduction in H3K27me3 levels is observed across the genome upon EZH2 inhibitor treatment.
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| Contributor(s) |
Egan B, Craske M, Labhart P, Yuan C, Guler GD, Arnott D, Maille T, Busby J, Henry C, Kelly TK, Tindell C, Jhunjhunwala S, Zhao F, Hatton C, Bryant BM, Classon M, Trojer P |
| Citation(s) |
27875550 |
| Submission date |
Dec 16, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Suchit Jhunjhunwala |
| E-mail(s) |
suchitj@gene.com
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| Organization name |
Genentech
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| Street address |
1 DNA Way, MS-444A
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA270645 |
| SRA |
SRP051259 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64243_RAW.tar |
4.6 Mb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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