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Series GSE64287 Query DataSets for GSE64287
Status Public on Nov 23, 2015
Title Role of IL-2 inducible T cell kinase (ITK) and Bruton's tyrosine kinase (BTK) in mast cell response to lipopolysaccharide (LPS)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mast cells are indispensable for LPS-induced septic hypothermia, in which TNF-α plays an essential role to initiate sepsis. Tec family non-receptor tyrosine kinases ITK and BTK regulate mast cell-derived TNF-α in response to allergic antigen, but their role in LPS-induced TNF-α production by mast cells and related pathology is unclear. We sought to investigate the role(s) of ITK and BTK in mast cell response in septic condition. We found that the absence of ITK and BTK leads to enhanced TNF-α production by bone marrow-derived mast cells (BMMC). Itk-/-Btk-/- mast cells exhibit hyperactive preformed and LPS-induced TNF-α production, along with enhanced expression of other related genes such as NF-κB targeted genes, compared to WT cells.
 
Overall design Bone marrow cells from 8-week old WT, Itk-/-, Btk-/- and Itk-/-Btk-/- (double knockout: DKO) C57Bl/6 mice were cultured in murine Interleukin-3/Stem cell factor (IL-3/SCF) supplemented medium for 5 weeks to derive mast cells. WT, Itk-/-, Btk-/- and DKO bone marrow-derived mast cells (BMMC) were factor starved in medium without IL-3/SCF for 12 hours, followed by treatment with PBS (control) or 100 ng/ml LPS for 1 hour. Triplicates of each group were subjected to mouse whole genome genechip microarray analysis. Replicates were randomized on different chips to avoid systematic error.
 
Contributor(s) Huang W, August A
Citation(s) 26581914, 27081634
Huang W, August A. Role(s) of IL-2 inducible T cell kinase and Bruton?s tyrosine kinase in mast cell response to lipopolysaccharide. Genomics Data. 2016;8:18-20. doi:10.1016/j.gdata.2016.02.010.
Submission date Dec 17, 2014
Last update date May 29, 2020
Contact name Weishan Huang
E-mail(s) wh296@cornell.edu
Phone 607-253-4014
Organization name Cornell University
Department Microbiology & Immunology
Lab Avery August
Street address C5 121, Cornell U.
City Ithaca
State/province New York
ZIP/Postal code 14853
Country USA
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (24)
GSM1568011 WT BMMC with PBS as control, biological rep1
GSM1568012 WT BMMC with PBS as control, biological rep2
GSM1568013 WT BMMC with PBS as control, biological rep3
Relations
BioProject PRJNA270640

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64287_Gene-level_analysis_quantile-normalized.txt.gz 4.2 Mb (ftp)(http) TXT
GSE64287_Probe-level_analysis_with_annotation.txt.gz 6.4 Mb (ftp)(http) TXT
GSE64287_RAW.tar 53.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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