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Status |
Public on Jan 01, 2015 |
Title |
IFNγ signaling endows DCs with the capacity to control type I inflammation during parasitic infection through promoting T-bet+ regulatory T cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Transcriptome analysis of IFNγ-insensitive DCs IFNγ signaling drives dendritic cells (DCs) to promote type I T cell (Th1) immunity. Here, we show that activation of DCs by IFNγ is equally crucial for the differentiation of a population of T-bet+ regulatory T (Treg) cells specialized to inhibit Th1 immune responses. Conditional deletion of IFNγ receptor in DCs but not in Treg cells resulted in a severe defect in this specific Treg cell subset, leading to exacerbated immune pathology during parasitic infections. Mechanistically, IFNγ-unresponsive DCs failed to produce sufficient amount of IL-27, a cytokine required for optimal T-bet induction in Treg cells. Thus, IFNγ signalling endows DCs with the ability to efficiently control a specific type of T cell immunity through promoting a corresponding Treg cell population.
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Overall design |
We analyzed 3 WT DC samples and 3 IFNγR2 KO DC samples isolated from unmanipulated mice. In addition, we analyzed 3 WT DC samples and 3 IFNγR2 KO DC samples isolated from mixed BM chimeras (WT + IFNgR2KO) day 8 T. gondii infected.
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Contributor(s) |
Lee H, Khan AA, Cho S, Lin L, Nguyen DT, Lu L |
Citation(s) |
25658840 |
Submission date |
Dec 31, 2014 |
Last update date |
Feb 21, 2018 |
Contact name |
Li-Fan Lu |
E-mail(s) |
lifanlu@ucsd.edu
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Organization name |
University of California, San Diego
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Department |
Biological Sciences
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Street address |
9500 Gilman Dr., NSB 5314
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City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
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Platforms (1) |
GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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Samples (12)
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Relations |
BioProject |
PRJNA271373 |