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Series GSE64594 Query DataSets for GSE64594
Status Public on Jan 01, 2015
Title IFNγ signaling endows DCs with the capacity to control type I inflammation during parasitic infection through promoting T-bet+ regulatory T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptome analysis of IFNγ-insensitive DCs
IFNγ signaling drives dendritic cells (DCs) to promote type I T cell (Th1) immunity. Here, we show that activation of DCs by IFNγ is equally crucial for the differentiation of a population of T-bet+ regulatory T (Treg) cells specialized to inhibit Th1 immune responses. Conditional deletion of IFNγ receptor in DCs but not in Treg cells resulted in a severe defect in this specific Treg cell subset, leading to exacerbated immune pathology during parasitic infections. Mechanistically, IFNγ-unresponsive DCs failed to produce sufficient amount of IL-27, a cytokine required for optimal T-bet induction in Treg cells. Thus, IFNγ signalling endows DCs with the ability to efficiently control a specific type of T cell immunity through promoting a corresponding Treg cell population.
 
Overall design We analyzed 3 WT DC samples and 3 IFNγR2 KO DC samples isolated from unmanipulated mice. In addition, we analyzed 3 WT DC samples and 3 IFNγR2 KO DC samples isolated from mixed BM chimeras (WT + IFNgR2KO) day 8 T. gondii infected.
 
Contributor(s) Lee H, Khan AA, Cho S, Lin L, Nguyen DT, Lu L
Citation(s) 25658840
Submission date Dec 31, 2014
Last update date Feb 21, 2018
Contact name Li-Fan Lu
E-mail(s) lifanlu@ucsd.edu
Organization name University of California, San Diego
Department Biological Sciences
Street address 9500 Gilman Dr., NSB 5314
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (12)
GSM1575119 WT_DC_rep1
GSM1575120 WT_DC_rep2
GSM1575121 WT_DC_rep3
Relations
BioProject PRJNA271373

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64594_RAW.tar 103.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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