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Status |
Public on Dec 08, 2006 |
Title |
Gene expression profiles of P1 and adult bladders isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 11) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the adult and postnatal day 1 bladder. Keywords: Gene expression comparison between postnatal day 1 and adult bladders
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Overall design |
Bladders, from postnatal day 1 and adult transgenic mice in which EGFP is driven by a SMGA promoter/EGFP, were isolated by dissection and total RNA was extracted and underwent 2-round amplification (Epicentre® Biotechnologies) for gene expression analysis using the Affymetrix MOE430 microarray chip
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Contributor(s) |
Lessard J, Szucsik JC |
Citation missing |
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Submission date |
Dec 06, 2006 |
Last update date |
Feb 11, 2019 |
Contact name |
GUDMAP Developers |
E-mail(s) |
gudmap-db@gudmap.org
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Phone |
+44 131 651 8500
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Organization name |
IGMM MRC Human Genetics Unit
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Lab |
GUDMAP Database Group
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Street address |
Crewe Road
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City |
Edinburgh |
ZIP/Postal code |
EH4 2XU |
Country |
United Kingdom |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (7)
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GSM148652 |
Postnatal day 1 male bladder J_13B |
GSM148653 |
Postnatal day 1 male bladder J_15B |
GSM148654 |
Postnatal day 1 male bladder J_40B |
GSM148655 |
Postnatal day 1 male and female bladders J_Ref_1 |
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Relations |
BioProject |
PRJNA98699 |