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| Status |
Public on Dec 09, 2007 |
| Title |
CA/AA time course of y w; tim01 |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Circadian clocks are temporally aligned to the environment via signals, or Zeitgebers, such as daily light and temperature cycles, food availability, and social behavior. In this study, we show that genome-wide expression profiles from temperature-entrained flies show a dramatic difference in the presence or absence of a thermocycle. Whereas transcription appears to be modified globally by changes in temperature, there is a specific set of transcripts that continue to oscillate in constant conditions following temperature entrainment. These transcripts show a significant overlap with a previously defined set of transcripts oscillating in response to a photocycle. Further, these overlapping transcripts maintain the same mutual phase relationships after entrainment by temperature or light. Comparison of the collective temperature- and light-entrained circadian phases indicates that natural environmental light and temperature cycles cooperatively entrain the circadian clock. These findings suggest that a single transcriptional clock in the adult fly head is able to integrate information from both light and temperature.
Keywords: circadian time course
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| Overall design |
y w; tim01 flies that had been reared in constant darkness initially at 25 C and later in a 12-hr 18 C/ 12-hr 25 C thermocycle for more than 4 days were harvested every four hours during an additional 18 C/25 C thermo cycle (also indicated as a cold/ambient or CA cycle) or an additional day at constant 25 C (also indicated as ambient/ambient or AA) . Relative to time CA0 as the time of the onset of the 18 C cryophase or AA0 as the time of the onset of the subjective cryophase during constant 25 C, samples were collected in a CA2-
CA6-CA10-CA14-CA18-CA22 and AA2-AA6-AA10-AA14-AA18-AA22 schedule. Heads
were isolated by breaking up frozen flies and passing them through a set of
sieves. RNA was prepared using guanidine-thiocyanate extraction followed by
purification over a CsCl gradient. Additional purification of the RNA samples was
achieved by applying them to Rneasy columns (Qiagen). Biotin-labeled cRNA
probe was generated from 25 μg of purified RNA and hybridized as described
previously (Wijnen H, Naef F, and Young MW, Methods Enzymol. 2005; 393: 341-365).
For more information see also http://biorhythm.rockefeller.edu
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| Contributor(s) |
Boothroyd C, Wijnen H, Naef F, Saez L, Young MW |
| Citation(s) |
17411344 |
| Submission date |
Dec 09, 2006 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Herman Wijnen |
| E-mail(s) |
hw3a11@soton.ac.uk
|
| Phone |
02380594336
|
| Organization name |
University of Southampton
|
| Department |
Centre for Biological Sciences
|
| Lab |
Wijnen
|
| Street address |
Life Sciences Building 85 - Highfield Campus
|
| City |
Southampton |
| State/province |
Hampshire |
| ZIP/Postal code |
SO17 1BJ |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL72 |
[DrosGenome1] Affymetrix Drosophila Genome Array |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA104227 |
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