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| Status |
Public on Oct 11, 2016 |
| Title |
Polycomb Underlies Transcriptional Heterogeneity in Lineage Priming of Embryonic Stem Cells [gene expression] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Quantitation of polyA mRNA levels in subpopulations of the HexVenus reporter (clone HV5.1) mouse ES cell line growing under self-renewing conditions. Subpopulations were identified and isolated based on the expression of the ES cell surface marker SSEA 1 and the expression of the venus protein. At approximately 70% confluence, cells were trypsinised, resuspended in FACs buffer (10%FCS in PBS) and incubated with a mouse monoclonal antibody to SSEA 1 (MC-480, Developmental Hybridoma Studies Bank, University of Iowa). Cells were then incubated with an Alexa-647 conjugated anti-mouse IgM antibody (Invitrogen) and subpopulations were fractionated by flow cytometry. The aim was to identify genes which are differentially expressed between FACS-sorted HV-S+ and HV+S+ primed mESC populations.
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| Overall design |
mESCs carrying a fluorescently tagged Hhex-Venus (HV) reporter construct were FACS sorted into Ssea1+HV- and Ssea1+HV+ populations. RNA was isolated from these populations immediately following FACS sorting and hybridised to Agilent 44k expression arrays. This process was performed as two independent biological replicates.
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| Contributor(s) |
Illingworth RS, Hölzenspies JJ, Bickmore WA, Brickman JM |
| Citation(s) |
27723457 |
| Submission date |
Jan 13, 2015 |
| Last update date |
May 10, 2018 |
| Contact name |
Rob S Illingworth |
| E-mail(s) |
robert.illingworth@ed.ac.uk
|
| Phone |
01316519640
|
| Organization name |
The University of Edinburgh
|
| Department |
Centre for regenerative Medicine
|
| Lab |
Illingworth
|
| Street address |
Centre for Regenerative Medicine
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE65289 |
Polycomb Underlies Transcriptional Heterogeneity in Lineage Priming of Embryonic Stem Cells |
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| Relations |
| BioProject |
PRJNA272538 |
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