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Series GSE64942 Query DataSets for GSE64942
Status Public on Mar 27, 2015
Title Piwi proteins and piRNAs in mammalian oocytes and early embryos
Organisms Macaca fascicularis; Homo sapiens; Bos taurus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Germ cells of most animals critically depend on piRNAs and Piwi proteins. Surprisingly, piRNAs in mouse oocytes are relatively rare and dispensable. We present compelling evidence for strong Piwi-piRNA expression in oocytes of other mammals. Human fetal oocytes express PIWIL2 and transposon-enriched piRNAs. Oocytes in adult human ovary express PIWIL1 and PIWIL2, while those in bovine ovary just express PIWIL1. In human, macaque and bovine ovaries we find piRNAs that resemble testis-borne pachytene piRNAs. Isolated bovine follicular oocytes were shown to contain abundant, relatively short piRNAs that preferentially target transposable elements. Using label-free quantitative proteome analysis we show that these maturing oocytes strongly and specifically express the thus-far uncharacterized PIWIL3 protein, alongside other known piRNA-pathway components. In bovine early embryos these piRNAs are still abundant, revealing a potential impact of piRNAs on mammalian embryogenesis. Our results reveal unexpected, highly dynamic piRNA pathways in mammalian oocytes and early embryos.
 
Overall design Analyses of multiple small RNA libraries obtained from fetal/adult oocytes, cumulus cells, ovary, testis and 2-4 cell stage ivf embryos of multiple mammalian species.
 
Contributor(s) Rovers E, Rosenkranz D, Mahdipour M, Han C, He N, Chuva de Sousa Lopes S, van der Westerlaken L, Zischler H, Butter F, Roelen B, Ketting RF
Citation(s) 25818294, 26484274
Submission date Jan 13, 2015
Last update date May 15, 2019
Contact name David Rosenkranz
E-mail(s) rosenkranz@uni-mainz.de
Organization name Johannes Gutenberg-University Mainz
Department Institute of Anthropology
Street address Anselm-Franz-von-Bentzel-Weg 7
City Mainz
ZIP/Postal code 55099
Country Germany
 
Platforms (3)
GPL15520 Illumina MiSeq (Homo sapiens)
GPL19646 Illumina MiSeq (Bos taurus)
GPL19647 Illumina MiSeq (Macaca fascicularis)
Samples (41)
GSM1584495 Bovine_GV_a
GSM1584496 Bovine_GV_b1
GSM1584497 Bovine_MI_1
Relations
BioProject PRJNA272542
SRA SRP052074

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Supplementary file Size Download File type/resource
GSE64942_RAW.tar 489.6 Mb (http)(custom) TAR (of FASTA)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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