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Series GSE65018 Query DataSets for GSE65018
Status Public on Mar 31, 2015
Title The epithelial cell transcriptome after alpha-toxin treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Responsiveness of cells to alpha-toxin (Hla) from Staphylococcus aureus appears to occur in a cell-type dependent manner. Here, we compare two human bronchial epithelial cell lines, i.e. Hla-susceptible 16HBE14o- and Hla-resistant S9 cells, by a quantitative multi-omics strategy for a better understanding of Hla-induced cellular programs. Phosphoproteomics revealed a substantial impact on phosphorylation-dependent signaling in both cell models and highlights alterations in signaling pathways associated with cell-cell and cell-matrix contacts as well as the actin cytoskeleton as key features of early rHla-induced effects. Along comparable changes in down-stream activity of major protein kinases significant differences between both models were found upon rHla-treatment including activation of EGFR and MAPK1/3 signaling in S9 and repression in 16HBE14o- cells. System-wide transcript and protein expression profiling indicate induction of an immediate early response in either model. In addition, EGFR and MAPK1/3-mediated changes in gene expression suggest cellular recovery and survival in S9 cells but cell death in 16HBE14o- cells. Strikingly, inhibition of the EGFR sensitized S9 cells to Hla indicating that the cellular capacity of activation of the EGFR is a major protective determinant against Hla-mediated cytotoxic effects.
 
Overall design Design includes two different human bronchial epithelial cell lines, one control treatment (mock treatment for 2 hours) and one condition (2 alpha toxin treatment for 2 hours). Experiments for both cell line were replicated twice (biological replication).
 
Contributor(s) Mostertz J, Chilukoti RK
Citation(s) 25816343
Submission date Jan 15, 2015
Last update date Jul 26, 2018
Contact name Joerg Mostertz
E-mail(s) mostertz@uni-greifswald.de
Organization name Greifswald University
Department Competence Center Functional Genomics
Lab Pathoproteomics
Street address F.-L.-Jahnstr 15
City Greifswald
ZIP/Postal code 17489
Country Germany
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (8)
GSM1585916 S9_KO_1
GSM1585917 S9_KO_2
GSM1585918 S9_HLA_1
Relations
BioProject PRJNA272720

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65018_RAW.tar 35.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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