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| Status |
Public on Dec 11, 2007 |
| Title |
Gene expression profiling in a congenic strain identifies candidate genes for nutrient intake QTL on mouse chromosome 17 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this experiment was to identify genes contributing to fat, carbohydrate, and total calorie intake in mice by comparing gene expression in liver and hypothalamus of an interval-specific congenic strain, B6.CAST-17, with that of the control strain. This congenic strain has previously confirmed genetic loci on Chr 17 linked to increased carbohydrate (Mnic1) and kilocalorie (Kcal2) intake.
Keywords: Comparative gene expression analysis
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| Overall design |
RNA obtained from six male B6.CAST-17 homozygous congenic mice and six male wild type B6 mice was pooled. Gene expression in hypothalamus and liver was compared between the strains under two diet conditions: 1) chow (LabDiet #5001) and 2) 78% carbohydrate/22% protein vs. 78% fat/22% protein, 4 h before lights out on day 2. Mice were selected based on phenotypic variation; specifically, 20-25 mice of each strain were tested in a self-selection diet protocol for 10 days. Mice that displayed the most divergent phenotypic values for nutrient intake (kcal per body weight) were chosen for study, returned to chow diet for 6 wks, and then returned to the 2-choice diet for one day.
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| Contributor(s) |
Kumar KG, Smith-Richards BK |
| Citation(s) |
19776624 |
| Submission date |
Dec 11, 2006 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Brenda Richards |
| E-mail(s) |
richarbk@pbrc.edu
|
| Organization name |
Pennington Biomedical Research Center
|
| Department |
Basic Science
|
| Lab |
Genetics of Eating Behavior
|
| Street address |
6400 Perkins Rd.
|
| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
|
| Samples (8)
|
| GSM148884 |
Brenda_Richards_ABI_Hypothalamus_C57BL/6J_Chow |
| GSM148886 |
Brenda_Richards_ABI_Hypothalamus_B6.CAST-17-congenics_Chow |
| GSM148891 |
Brenda_Richards_Hypothalamus_C57BL/6J_2 choice diet |
| GSM148893 |
Brenda_Richards_Hypothalamus_2choice diet_B6.CAST-17 congenics |
| GSM148895 |
Brenda_Richards_Liver_2choice diet_B6.CAST-17 congenics |
| GSM148896 |
Brenda_Richards_Liver_2choice diet_C57BL/6J |
| GSM148898 |
Brenda_Richards_Liver_B6.CAST-17congenics_Chow |
| GSM148899 |
Brenda_Richards_Liver_Chow_C57BL/6J |
|
| Relations |
| BioProject |
PRJNA98683 |
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